Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | PfiT-PfiA/ParE-Phd |
| Location | 6807..7418 | Replicon | plasmid pUSA011-2 |
| Accession | NZ_CP045801 | ||
| Organism | Pseudomonas syringae USA011 | ||
Toxin (Protein)
| Gene name | PfiT | Uniprot ID | - |
| Locus tag | N028_RS27015 | Protein ID | WP_024639831.1 |
| Coordinates | 6807..7157 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | PfiA | Uniprot ID | A0A0P9SUR8 |
| Locus tag | N028_RS27020 | Protein ID | WP_024639832.1 |
| Coordinates | 7170..7418 (-) | Length | 83 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| N028_RS26975 | 1877..2215 | - | 339 | WP_153795706.1 | conjugal transfer protein | - |
| N028_RS26980 | 2212..2919 | - | 708 | WP_003436811.1 | lytic transglycosylase domain-containing protein | - |
| N028_RS26985 | 2932..3174 | - | 243 | WP_003436809.1 | hypothetical protein | - |
| N028_RS26990 | 3263..3775 | - | 513 | WP_003436807.1 | hypothetical protein | - |
| N028_RS26995 | 3992..4387 | + | 396 | WP_024639869.1 | helix-turn-helix domain-containing protein | - |
| N028_RS27000 | 4399..4596 | - | 198 | WP_003436803.1 | hypothetical protein | - |
| N028_RS27005 | 4695..5795 | - | 1101 | WP_024639870.1 | polyamine ABC transporter substrate-binding protein | - |
| N028_RS27010 | 5904..6677 | + | 774 | WP_005782492.1 | LuxR family transcriptional regulator | - |
| N028_RS27015 | 6807..7157 | - | 351 | WP_024639831.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| N028_RS27020 | 7170..7418 | - | 249 | WP_024639832.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| N028_RS27025 | 7669..8031 | + | 363 | WP_152981388.1 | hypothetical protein | - |
| N028_RS27030 | 8072..9034 | + | 963 | WP_032611494.1 | tyrosine-type recombinase/integrase | - |
| N028_RS27035 | 9060..9380 | - | 321 | WP_024639835.1 | hypothetical protein | - |
| N028_RS27040 | 9790..10179 | + | 390 | WP_024639836.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| N028_RS27045 | 10227..10538 | - | 312 | WP_024639837.1 | helix-turn-helix domain-containing protein | - |
| N028_RS27050 | 11128..11769 | - | 642 | WP_024639838.1 | SOS response-associated peptidase | - |
| N028_RS27055 | 12007..12294 | - | 288 | WP_024639839.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..43372 | 43372 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12809.69 Da Isoelectric Point: 4.3696
>T142604 WP_024639831.1 NZ_CP045801:c7157-6807 [Pseudomonas syringae USA011]
MNTSALRFTDLAQQSLEDQVEHLAVTQGFSSAAQRIGTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITVIVVLLVLGGKQSVEQALIRYCLLQPI
MNTSALRFTDLAQQSLEDQVEHLAVTQGFSSAAQRIGTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRIFY
EVMDSDGITVIVVLLVLGGKQSVEQALIRYCLLQPI
Download Length: 351 bp
>T142604 NZ_CP060748:c3616918-3616520 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 83 a.a. Molecular weight: 9375.74 Da Isoelectric Point: 9.8452
>AT142604 WP_024639832.1 NZ_CP045801:c7418-7170 [Pseudomonas syringae USA011]
MRVETISYLKRNAADLPLDEPLIVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFA
HK
MRVETISYLKRNAADLPLDEPLIVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSTDELKARLSRRFA
HK
Download Length: 249 bp
>AT142604 NZ_CP060748:c3617214-3616921 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|