Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 976807..977441 Replicon chromosome
Accession NZ_CP045799
Organism Pseudomonas syringae USA011

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag N028_RS04520 Protein ID WP_024640186.1
Coordinates 976807..977211 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3M5LBD7
Locus tag N028_RS04525 Protein ID WP_024640185.1
Coordinates 977211..977441 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N028_RS04480 971819..972301 + 483 WP_024640189.1 PAS domain-containing protein -
N028_RS04485 972398..973654 + 1257 WP_003404790.1 3-phosphoshikimate 1-carboxyvinyltransferase -
N028_RS04490 973802..974056 + 255 WP_003404793.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
N028_RS04495 974053..974400 + 348 WP_003404797.1 type II toxin-antitoxin system ChpB family toxin -
N028_RS04500 974448..975110 - 663 WP_003404800.1 ChrR family anti-sigma-E factor -
N028_RS04505 975110..975622 - 513 WP_003345995.1 sigma-70 family RNA polymerase sigma factor -
N028_RS04510 975858..976046 - 189 WP_024640188.1 hypothetical protein -
N028_RS04515 976045..976620 + 576 WP_024640187.1 hypothetical protein -
N028_RS04520 976807..977211 - 405 WP_024640186.1 tRNA(fMet)-specific endonuclease VapC Toxin
N028_RS04525 977211..977441 - 231 WP_024640185.1 antitoxin Antitoxin
N028_RS04530 977558..978439 - 882 WP_024640184.1 aldose 1-epimerase -
N028_RS04535 978414..979385 - 972 WP_024640183.1 DMT family transporter -
N028_RS04540 979470..980750 - 1281 WP_153795649.1 TRAP transporter large permease -
N028_RS04545 980751..981278 - 528 WP_003408544.1 TRAP transporter small permease -
N028_RS04550 981341..982366 - 1026 WP_024640181.1 TRAP transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 967033..977441 10408


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(7-34)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14872.13 Da        Isoelectric Point: 6.4779

>T142602 WP_024640186.1 NZ_CP045799:c977211-976807 [Pseudomonas syringae USA011]
MLKYMLDTNICIFTIKNKPASVREAFNLHHGQLCISAITLMELVYGAEKSLSPERNLAVVEGFTARLEVLPYDSDAAAHT
GMIRAELARAGTPIGPYDQMIAGHARSLGLVVITNNQREFQRVEGLRVEDWVSQ

Download         Length: 405 bp

>T142602 NZ_CP060748:2591328-2591435 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8535.55 Da        Isoelectric Point: 4.7247

>AT142602 WP_024640185.1 NZ_CP045799:c977441-977211 [Pseudomonas syringae USA011]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGENVSDDFMAERGQPAEQQRESL

Download         Length: 231 bp

>AT142602 NZ_CP060748:c2591272-2591216 [Escherichia coli]
TCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A3M5LBD7

References