Detailed information of TA system TA142600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 1924683..1925313
• Replicon: chromosome
• Accession: NZ_CP045798
• Organism: Thermoanaerosceptrum fracticalcis strain DRI-13

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A7G6E3D5
• Locus tag: BR63_RS09900
• Protein ID: WP_034421612.1
• Coordinates: 1924683..1925036 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: BR63_RS09905
• Protein ID: WP_034421614.1
• Coordinates: 1925038..1925313 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BR63_RS09885	1920224..1921051	-	828	WP_034421607.1	hypothetical protein	-
BR63_RS09890	1921261..1923411	-	2151	WP_034421609.1	RNA-binding transcriptional accessory protein	-
BR63_RS09895	1923425..1924429	-	1005	WP_034421610.1	asparaginase	-
BR63_RS09900	1924683..1925036	-	354	WP_034421612.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BR63_RS09905	1925038..1925313	-	276	WP_034421614.1	ribbon-helix-helix protein, CopG family	Antitoxin
BR63_RS09910	1925408..1926538	-	1131	WP_034421655.1	alanine racemase	-
BR63_RS09915	1926729..1928279	-	1551	WP_034421615.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
BR63_RS09920	1928267..1928653	-	387	WP_034421617.1	holo-ACP synthase	-
BR63_RS09925	1928763..1930100	-	1338	WP_034421618.1	phosphoglucosamine mutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (7-43)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 12728.67 Da
• Isoelectric Point: 5.1528

>T142600 WP_034421612.1 NZ_CP045798:c1925036-1924683 [Thermoanaerosceptrum fracticalcis]
MVTVKRGDIFYADLSPVVGSEQGGTRPVLIIQNDIGNQYSPTTIVAAITSQLDKAKLPTHVEISCESCGLTKDSVILTEQ
IRTIDKRRLQEKVSSLDEVTMGKVSRALEISLGLVEL

Nucleotide

• Length: 354 bp

>T142600 NZ_CP060748:c2430484-2430308 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10835.65 Da
• Isoelectric Point: 4.9231

>AT142600 WP_034421614.1 NZ_CP045798:c1925313-1925038 [Thermoanaerosceptrum fracticalcis]
VSETKRIMICLPADLLEEVDGIMTIEKKNRSEFIREAMRFYLAETKRKNLREQMRRGYLEMAQINLTLAQEAFYSELEVD
ILYQNKLVECL

Nucleotide

• Length: 276 bp

>AT142600 NZ_CP060748:c2430262-2429846 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G6E3D5