Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 54945..55520 Replicon chromosome
Accession NZ_CP045798
Organism Thermoanaerosceptrum fracticalcis strain DRI-13

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G6DYK1
Locus tag BR63_RS00320 Protein ID WP_034424525.1
Coordinates 55191..55520 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G6DYK0
Locus tag BR63_RS00315 Protein ID WP_034424522.1
Coordinates 54945..55190 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BR63_RS00285 50081..51318 + 1238 Protein_54 IS110 family transposase -
BR63_RS00290 51559..52236 + 678 WP_034424510.1 C39 family peptidase -
BR63_RS00295 52332..52826 + 495 WP_034424513.1 hypothetical protein -
BR63_RS00300 52976..53113 + 138 WP_153802153.1 hypothetical protein -
BR63_RS00305 53296..53688 + 393 WP_034424515.1 hypothetical protein -
BR63_RS00310 54214..54648 + 435 WP_034424520.1 ACT domain-containing protein -
BR63_RS00315 54945..55190 + 246 WP_034424522.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BR63_RS00320 55191..55520 + 330 WP_034424525.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BR63_RS00325 55638..56012 - 375 WP_051966099.1 hypothetical protein -
BR63_RS00330 55990..56358 - 369 WP_034424527.1 hypothetical protein -
BR63_RS00335 56866..57396 + 531 WP_207724742.1 ECF transporter S component -
BR63_RS00340 57383..58141 + 759 WP_034424530.1 hypothetical protein -
BR63_RS00345 58627..58944 + 318 WP_051966101.1 NapC/NirT family cytochrome c -
BR63_RS00350 58941..59915 + 975 WP_051966103.1 ammonia-forming cytochrome c nitrite reductase subunit c552 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-98)

Antitoxin

Antitoxin PemI-like

(1-80)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12285.18 Da        Isoelectric Point: 8.2644

>T142599 WP_034424525.1 NZ_CP045798:55191-55520 [Thermoanaerosceptrum fracticalcis]
MGYIPKKGDMVWLDFTPQSGHEQKGRRPAIVISNDFFNNKTGLAVVCPITSSRRDYPLHVEISGCKKVTGYIMVEQVKSV
DYLARGAELIERAPETVINETLSRFFACF

Download         Length: 330 bp

>T142599 NZ_CP060748:2357589-2358911 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9433.58 Da        Isoelectric Point: 4.8430

>AT142599 WP_034424522.1 NZ_CP045798:54945-55190 [Thermoanaerosceptrum fracticalcis]
MITRFQKWGNSQGIRIPKHILNQASFSEEDEVEIVVEDKSIVIRHVISPLKKYDLKELFDGYNGDYRPGEDWGSPQGREE
L

Download         Length: 246 bp

>AT142599 NZ_CP060748:2357323-2357589 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G6DYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A7G6DYK0

References