Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 145309..145868 Replicon plasmid p1CFSAN029786
Accession NZ_CP045785
Organism Shigella dysenteriae strain CFSAN029786

Toxin (Protein)


Gene name relE Uniprot ID D2AJT2
Locus tag GH770_RS25205 Protein ID WP_000421262.1
Coordinates 145309..145584 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q327A8
Locus tag GH770_RS25210 Protein ID WP_001178088.1
Coordinates 145584..145868 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GH770_RS25175 (GH770_25175) 140311..141524 + 1214 WP_167544920.1 IS3 family transposase -
GH770_RS25185 (GH770_25185) 141588..142718 + 1131 WP_050546159.1 IS91 family transposase -
GH770_RS25190 (GH770_25190) 142913..144004 - 1092 Protein_177 IS66 family transposase -
GH770_RS25195 (GH770_25195) 144012..144579 + 568 Protein_178 plasmid segregation protein ParM -
GH770_RS26660 144582..144764 + 183 WP_241547534.1 plasmid partitioning/stability family protein -
GH770_RS26010 145040..145177 + 138 WP_000766699.1 hypothetical protein -
GH770_RS25205 (GH770_25205) 145309..145584 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GH770_RS25210 (GH770_25210) 145584..145868 - 285 WP_001178088.1 ribbon-helix-helix domain-containing protein Antitoxin
GH770_RS25215 (GH770_25215) 146320..146571 + 252 WP_000433928.1 transporter -
GH770_RS25220 (GH770_25220) 146621..146830 + 210 Protein_184 peptidoglycan-binding protein -
GH770_RS26665 146912..147013 - 102 Protein_185 ATP-binding protein -
GH770_RS25225 (GH770_25225) 147165..147521 + 357 WP_005041107.1 transposase -
GH770_RS25230 (GH770_25230) 147518..147868 + 351 WP_000624671.1 IS66 family insertion sequence element accessory protein TnpB -
GH770_RS25235 (GH770_25235) 147899..149491 + 1593 WP_046891721.1 IS66 family transposase -
GH770_RS26015 149498..149629 - 132 Protein_189 ATP-binding protein -
GH770_RS25240 (GH770_25240) 149848..150522 + 675 WP_046891833.1 IS66-like element accessory protein TnpA -
GH770_RS25245 (GH770_25245) 150519..150683 + 165 Protein_191 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC3 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ipaH1.4 / ospE2 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ 1..223637 223637
- inside IScluster/Tn - ospG / ipaH9.8 127607..170674 43067


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10766.48 Da        Isoelectric Point: 9.9324

>T142593 WP_000421262.1 NZ_CP045785:c145584-145309 [Shigella dysenteriae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T142593 NZ_CP060748:800676-800972 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10497.93 Da        Isoelectric Point: 6.2150

>AT142593 WP_001178088.1 NZ_CP045785:c145868-145584 [Shigella dysenteriae]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR

Download         Length: 285 bp

>AT142593 NZ_CP060748:800974-801369 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PVM3

Antitoxin

Source ID Structure
AlphaFold DB Q327A8

References