Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 137490..138015 Replicon plasmid p1CFSAN029786
Accession NZ_CP045785
Organism Shigella dysenteriae strain CFSAN029786

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag GH770_RS25155 Protein ID WP_001159860.1
Coordinates 137490..137795 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag GH770_RS25160 Protein ID WP_000813626.1
Coordinates 137797..138015 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GH770_RS26650 133331..133483 + 153 Protein_164 hypothetical protein -
GH770_RS25130 (GH770_25130) 133535..134231 + 697 Protein_165 IS1 family transposase -
GH770_RS25135 (GH770_25135) 134249..134731 - 483 Protein_166 type III secretion system effector protein kinase OspG -
GH770_RS26655 134814..134983 - 170 Protein_167 type II toxin-antitoxin system toxin YacB -
GH770_RS25140 (GH770_25140) 134983..135252 - 270 WP_000079956.1 type II toxin-antitoxin system antitoxin YacA -
GH770_RS25145 (GH770_25145) 135460..137097 - 1638 WP_000936802.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
GH770_RS25150 (GH770_25150) 137359..137466 - 108 WP_023592908.1 transposase domain-containing protein -
GH770_RS25155 (GH770_25155) 137490..137795 - 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
GH770_RS25160 (GH770_25160) 137797..138015 - 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GH770_RS25165 (GH770_25165) 138259..139487 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
GH770_RS25170 (GH770_25170) 139944..140216 + 273 WP_000019158.1 hypothetical protein -
GH770_RS25175 (GH770_25175) 140311..141524 + 1214 WP_167544920.1 IS3 family transposase -
GH770_RS25185 (GH770_25185) 141588..142718 + 1131 WP_050546159.1 IS91 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC3 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ipaH1.4 / ospE2 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ 1..223637 223637
- inside IScluster/Tn - ospG / ipaH9.8 127607..170674 43067


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T142592 WP_001159860.1 NZ_CP045785:c137795-137490 [Shigella dysenteriae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T142592 NZ_CP060748:739245-739559 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT142592 WP_000813626.1 NZ_CP045785:c138015-137797 [Shigella dysenteriae]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT142592 NZ_CP060748:739556-739972 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References