Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 103068..103843 Replicon plasmid p1CFSAN029786
Accession NZ_CP045785
Organism Shigella dysenteriae strain CFSAN029786

Toxin (Protein)


Gene name tacT Uniprot ID Q326V1
Locus tag GH770_RS24955 Protein ID WP_000405248.1
Coordinates 103068..103550 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q7BEG8
Locus tag GH770_RS24960 Protein ID WP_010921625.1
Coordinates 103541..103843 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GH770_RS26000 99512..99649 + 138 WP_005026553.1 hypothetical protein -
GH770_RS24930 (GH770_24930) 99931..100083 - 153 Protein_125 IS3 family transposase -
GH770_RS24935 (GH770_24935) 100139..100831 + 693 WP_000625268.1 type III secretion system effector cysteine methyltransferase OspZ -
GH770_RS24940 (GH770_24940) 100855..102022 - 1168 Protein_127 IS3 family transposase -
GH770_RS24945 (GH770_24945) 102096..102434 + 339 Protein_128 integrase core domain-containing protein -
GH770_RS24950 (GH770_24950) 102477..103000 - 524 Protein_129 transposase -
GH770_RS24955 (GH770_24955) 103068..103550 - 483 WP_000405248.1 GNAT family N-acetyltransferase Toxin
GH770_RS24960 (GH770_24960) 103541..103843 - 303 WP_010921625.1 DUF1778 domain-containing protein Antitoxin
GH770_RS24965 (GH770_24965) 104115..104387 + 273 WP_000019158.1 hypothetical protein -
GH770_RS24970 (GH770_24970) 104368..105576 + 1209 WP_153933146.1 IS91-like element ISSbo1 family transposase -
GH770_RS24975 (GH770_24975) 105748..105876 + 129 Protein_134 IS5/IS1182 family transposase -
GH770_RS24980 (GH770_24980) 106025..107479 - 1455 WP_000701106.1 type 3 secretion system effector OspC2 -
GH770_RS24985 (GH770_24985) 107904..108748 + 845 WP_153933147.1 IS5-like element ISSfl7 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC3 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ipaH1.4 / ospE2 / ospI / ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 / ospG / ipaH9.8 / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ 1..223637 223637
- inside IScluster/Tn - ipgB2 / ospD1 / ospF / ospD2 / ospB / icsP/sopA / nleE / ospC2 71285..112526 41241


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(29-149)

Antitoxin

Vibrio phage ICP1, Orf50

(19-94)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17736.50 Da        Isoelectric Point: 7.0382

>T142590 WP_000405248.1 NZ_CP045785:c103550-103068 [Shigella dysenteriae]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Download         Length: 483 bp

>T142590 NZ_CP060748:258535-258822 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11689.28 Da        Isoelectric Point: 4.9167

>AT142590 WP_010921625.1 NZ_CP045785:c103843-103541 [Shigella dysenteriae]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Download         Length: 303 bp

>AT142590 NZ_CP060748:258809-259111 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NWA7

Antitoxin

Source ID Structure
AlphaFold DB Q7BEG8

References