Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3009010..3009593 Replicon chromosome
Accession NZ_CP045784
Organism Shigella dysenteriae strain CFSAN029786

Toxin (Protein)


Gene name mazF Uniprot ID Q31XK8
Locus tag GH770_RS15245 Protein ID WP_000254733.1
Coordinates 3009258..3009593 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag GH770_RS15240 Protein ID WP_000581937.1
Coordinates 3009010..3009258 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GH770_RS15230 (3005349) 3005349..3006650 + 1302 WP_000046825.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
GH770_RS15235 (3006698) 3006698..3008932 + 2235 WP_000226815.1 GTP pyrophosphokinase -
GH770_RS15240 (3009010) 3009010..3009258 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GH770_RS15245 (3009258) 3009258..3009593 + 336 WP_000254733.1 endoribonuclease MazF Toxin
GH770_RS15250 (3009664) 3009664..3010455 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
GH770_RS15255 (3010678) 3010678..3012315 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GH770_RS15260 (3012403) 3012403..3013701 + 1299 WP_000036723.1 phosphopyruvate hydratase -
GH770_RS15265 (3013761) 3013761..3014087 - 327 Protein_2994 YgcG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2971504..3017539 46035


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12167.15 Da        Isoelectric Point: 8.7218

>T142576 WP_000254733.1 NZ_CP045784:3009258-3009593 [Shigella dysenteriae]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T142576 NZ_CP060744:78964-79347 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT142576 WP_000581937.1 NZ_CP045784:3009010-3009258 [Shigella dysenteriae]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT142576 NZ_CP060744:78722-78964 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTACTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31XK8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References