Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2603016..2603670 Replicon chromosome
Accession NZ_CP045784
Organism Shigella dysenteriae strain CFSAN029786

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GH770_RS13195 Protein ID WP_000244777.1
Coordinates 2603016..2603423 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GH770_RS13200 Protein ID WP_000354046.1
Coordinates 2603404..2603670 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GH770_RS13175 (2598973) 2598973..2600706 - 1734 WP_000813209.1 single-stranded-DNA-specific exonuclease RecJ -
GH770_RS13180 (2600712) 2600712..2601422 - 711 WP_000715205.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GH770_RS13185 (2601447) 2601447..2602343 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GH770_RS13190 (2602455) 2602455..2602976 + 522 WP_001055874.1 flavodoxin FldB -
GH770_RS13195 (2603016) 2603016..2603423 - 408 WP_000244777.1 protein YgfX Toxin
GH770_RS13200 (2603404) 2603404..2603670 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GH770_RS13205 (2603913) 2603913..2604893 + 981 WP_000886098.1 tRNA-modifying protein YgfZ -
GH770_RS13210 (2604970) 2604970..2605629 - 660 WP_000250274.1 hemolysin III family protein -
GH770_RS13215 (2605793) 2605793..2606104 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
GH770_RS13220 (2606149) 2606149..2607582 + 1434 WP_004986559.1 6-phospho-beta-glucosidase BglA -
GH770_RS13225 (2607639) 2607639..2608382 - 744 WP_000951947.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T142574 WP_000244777.1 NZ_CP045784:c2603423-2603016 [Shigella dysenteriae]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T142574 NZ_CP060737:c3968113-3967703 [Enterobacter roggenkampii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGTCTGGTTGCGGC
CTTTGTGTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGTTACTGTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATCAAGTTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGCTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTTTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTATTGCAACAGACGA
CGCAGGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT142574 WP_000354046.1 NZ_CP045784:c2603670-2603404 [Shigella dysenteriae]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT142574 NZ_CP060737:c3968360-3968094 [Enterobacter roggenkampii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGACGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References