Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4300485..4301104 Replicon chromosome
Accession NZ_CP045783
Organism Klebsiella variicola strain LEMB11

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GFC07_RS20535 Protein ID WP_002892050.1
Coordinates 4300485..4300703 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag GFC07_RS20540 Protein ID WP_008805436.1
Coordinates 4300730..4301104 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFC07_RS20505 4296528..4296791 + 264 WP_008805431.1 type B 50S ribosomal protein L31 -
GFC07_RS20510 4296791..4296931 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GFC07_RS20515 4296928..4297626 - 699 WP_100781115.1 GNAT family N-acetyltransferase -
GFC07_RS20520 4297727..4299184 + 1458 WP_008805433.1 PLP-dependent aminotransferase family protein -
GFC07_RS20525 4299153..4299623 - 471 WP_008805434.1 YlaC family protein -
GFC07_RS26700 4299660..4299788 + 129 Protein_4035 hypothetical protein -
GFC07_RS20530 4299760..4300326 - 567 WP_008805435.1 maltose O-acetyltransferase -
GFC07_RS20535 4300485..4300703 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GFC07_RS20540 4300730..4301104 - 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
GFC07_RS20545 4301587..4304733 - 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit -
GFC07_RS20550 4304756..4305949 - 1194 WP_008805438.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T142565 WP_002892050.1 NZ_CP045783:c4300703-4300485 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T142565 NZ_CP060737:45676-46074 [Enterobacter roggenkampii]
ATGAGACATATGCTGGATACGAATATTGTGAGTCACTTGTTTAAACGACACCCGGAGGTTGTAAGCAGAATGACATGTCT
GGCGCCGGGTGACGTTTGTATCTCAAGCATTACCGAAGCGGAATTGCTGTATGGTGCTGACAAGAAGAAAAGCAGTAGAT
TGAAAGAAACAATCCGGGAGTTTCTCAATACCATCACGATTTGCGACTGGGACAGCGACGCCGCAGCAACGTACGGTGAA
CTCCGTGCAACCCTGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAACTGATCGCTGCACATGCTATCAGTCGGGG
AACAACGATCGTTACTAACGATCGTGCATTCAGAATGGTGCAGGAGCTTGCCGTCGAGGACTGGACGACAACGTTGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT142565 WP_008805436.1 NZ_CP045783:c4301104-4300730 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT142565 NZ_CP060737:45413-45679 [Enterobacter roggenkampii]
ATGGAACGCACAGCAAAACTATTCAAAAAGGGTCAGAATCAGGCCGTTATGCTGCCTGCTGAATTCGCGTTTGATACGGA
GAGCGTCTACATCCGACGGGATGAGGAGGGGAATGTCGTTTTGGCGGCAAAACCTGTTCTAATAAAGAGCTGGGATAATT
TTCTCCGCATGCTGGCAGAAACGGACGTACCAGATACGTTTTTGAGCAAGGAGGAACGCAATCAGAGTGTTACGACAAGA
GACCCTCTGGATGGAGCATGCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References