Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2674568..2675199 Replicon chromosome
Accession NZ_CP045783
Organism Klebsiella variicola strain LEMB11

Toxin (Protein)


Gene name hicA Uniprot ID A0A1F2M5P8
Locus tag GFC07_RS12945 Protein ID WP_023323362.1
Coordinates 2674924..2675199 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag GFC07_RS12940 Protein ID WP_004181489.1
Coordinates 2674568..2674927 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFC07_RS12915 2670825..2671769 + 945 WP_032733899.1 nickel ABC transporter permease subunit NikB -
GFC07_RS12920 2671766..2672599 + 834 WP_012967107.1 nickel ABC transporter permease subunit NikC -
GFC07_RS12925 2672599..2673363 + 765 WP_060876239.1 nickel import ATP-binding protein NikD -
GFC07_RS12930 2673360..2674151 + 792 WP_012540373.1 nickel import ATP-binding protein NikE -
GFC07_RS12935 2674139..2674540 + 402 WP_048330886.1 nickel-responsive transcriptional regulator NikR -
GFC07_RS12940 2674568..2674927 - 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GFC07_RS12945 2674924..2675199 - 276 WP_023323362.1 type II toxin-antitoxin system HicA family toxin Toxin
GFC07_RS12950 2675596..2676609 + 1014 WP_153690825.1 magnesium transporter -
GFC07_RS12955 2676644..2676919 - 276 WP_004204933.1 LysR family transcriptional regulator -
GFC07_RS12960 2677256..2678209 + 954 WP_012540370.1 hydroxyacid dehydrogenase -
GFC07_RS12965 2678220..2678918 + 699 WP_100781144.1 methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10260.86 Da        Isoelectric Point: 9.9665

>T142564 WP_023323362.1 NZ_CP045783:c2675199-2674924 [Klebsiella variicola]
MEQQVLSLRNKQHHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T142564 NZ_CP060734:44344-44829 [Enterobacter roggenkampii]
GTGGGACGTGTAACAGCACCAGAACCGTTAACAAGCTCCCATCAACTTACTGAGTTCGTCAGTGGCGAGGCTGTACTAGA
TGACTGGCTTAAACAGGGAGGATTAAAAAATCAGGCTCTCGGTTCAGCCCGAACGTTTGTTGTTTGTAAAACAGGCACGA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTAGAAGCAACGGGAAATATCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTCGATTTTTCATTCCGTGGTCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGATAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAGCTTTTATCTTCACCATGGCTTTAAGGCATCGAAAACTCAGGAACGGACATTATTTTTAAAGTTACCA
CAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT142564 WP_004181489.1 NZ_CP045783:c2674927-2674568 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT142564 NZ_CP060734:44090-44356 [Enterobacter roggenkampii]
ATGAAATCCGATATTCAGCTCAATCTTAGAGCCAAAGAATCGCAAAGAGCACTCATCGATGCTGCTGCAGAGATTCTCCA
TAAATCACGTACAGACTTCATTCTGGAAATGGCATGCCAGGCTGCGGAGAAGGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAGCAGTATGCAGAATTTATCGATATGCTCGATGCACCAGTTTCGGATGATGCTGCCATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M5P8

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References