Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3865950..3866760 Replicon chromosome
Accession NZ_CP045782
Organism Klebsiella pneumoniae strain LEMB18

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag GFB99_RS18630 Protein ID WP_002887280.1
Coordinates 3866227..3866760 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag GFB99_RS18625 Protein ID WP_002887278.1
Coordinates 3865950..3866216 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFB99_RS18605 3861822..3862166 - 345 WP_023287080.1 cation efflux system protein CusF -
GFB99_RS18610 3862184..3863569 - 1386 WP_040089708.1 efflux transporter outer membrane subunit -
GFB99_RS18615 3863741..3864424 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
GFB99_RS18620 3864414..3865847 + 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
GFB99_RS18625 3865950..3866216 + 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
GFB99_RS18630 3866227..3866760 + 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
GFB99_RS18635 3866808..3867929 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T142548 WP_002887280.1 NZ_CP045782:3866227-3866760 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T142548 NZ_CP060726:c4235623-4235189 [Providencia rettgeri]
ATGGCACTTGCTACTATAGTAATAGGTACTACATGGAGAGTAGTTGTGGCGTTCTATAAAATTTCCTCATTTAAGCGAAG
CACCAAAAAAATTAAGATAATGGATAAACACTTGCTTAAGGCTGCAAATGAAGTCTTAGCGGGGGTTTATGAAGCAGACT
TAGGAAGTGGTGTAATTAAAAAAAGGATTGCCATTAATGGAAAAGGGAAAAGTGGTGGGGTCAGGGTTATTATATTCTTC
AAAGTTAGCCATCATCTCTTTTTTGCCGATGGTTGGGAGAAATGCAATATAGCTGCTAAAGGAGTAAAAGAAATTGAAGA
TGATGAACTAGATGCGTATAAGAATTTATCTCGACTTTTTCTTAATTATACGGATGATAAAATTGCTGATTTAGTTTTAC
ATAATATTTTGGAGGAGCTTACTGATGAGCAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT142548 WP_002887278.1 NZ_CP045782:3865950-3866216 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT142548 NZ_CP060726:c4235199-4234870 [Providencia rettgeri]
ATGAGCAATAAACTAGAAAACATGCTTGAGCTAGCGAAAGAGTTACATCAGGTTGATGCTGCACCAAAAAGTTTAGTTAC
TGATTTAGAACAGAAGGTTGGCAATCGTAAAATAAATAATAAAATCAAGGTAGTACCTATGATGAGCGGTGCGCAAATCA
AACAACTCCGTGCTCAATATGGAATGTCACAAGCCATGTTAGCTTTAGCGCTGGGCATGTCTAAGGAAAGCGTATCTAAG
TGGGAAAGAGAAGAAAAAAAACCCAGTGGCCCAGCTCTAAGAATGCTACATCTTATAGAGCAGCGAGGGCCAGAGATTTT
AATTATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References