Detailed information of TA system TA142541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1734969..1735705
• Replicon: chromosome
• Accession: NZ_CP045782
• Organism: Klebsiella pneumoniae strain LEMB18

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GFB99_RS08415
• Protein ID: WP_032429294.1
• Coordinates: 1734969..1735451 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: GFB99_RS08420
• Protein ID: WP_003026799.1
• Coordinates: 1735439..1735705 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFB99_RS08390	1730369..1730947	+	579	WP_032432061.1	type 1 fimbrial protein	-
GFB99_RS08395	1730959..1731903	+	945	WP_077256325.1	fimbrial protein	-
GFB99_RS08400	1732116..1732715	+	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
GFB99_RS08405	1733166..1733357	+	192	WP_060589184.1	hypothetical protein	-
GFB99_RS08410	1733411..1734757	-	1347	WP_077264206.1	ISNCY family transposase	-
GFB99_RS08415	1734969..1735451	-	483	WP_032429294.1	GNAT family N-acetyltransferase	Toxin
GFB99_RS08420	1735439..1735705	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
GFB99_RS08425	1736150..1736380	-	231	WP_004206660.1	hypothetical protein	-
GFB99_RS08430	1736394..1736597	-	204	WP_004206661.1	hemolysin expression modulator Hha	-
GFB99_RS08435	1736658..1737149	-	492	WP_004206662.1	hypothetical protein	-
GFB99_RS08440	1737283..1738494	-	1212	WP_032415728.1	hypothetical protein	-
GFB99_RS08445	1738491..1738739	-	249	WP_004193994.1	hypothetical protein	-
GFB99_RS08450	1739097..1739447	+	351	WP_004206664.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
GFB99_RS08455	1739499..1739861	+	363	WP_004206665.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1723307..1735705	12398
	-	flank	IS/Tn	-	-	1733411..1734250	839

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17368.00 Da
• Isoelectric Point: 9.5822

>T142541 WP_032429294.1 NZ_CP045782:c1735451-1734969 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAAFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T142541 NZ_CP060725:3675257-3675625 [Sphingopyxis sp. OPL5]
ATGATCGACGCGCAGTTCGACAGCGACATTTTGATCGACGCCCTCAACGGCGTCGAGGCCGCGCGCAGCGAGATCCGCCG
CGCCGGGCGCAAGAGCGTCAGCCGGATCAGCTGGACCGAGGTGATGACCGCCGCCGACCCCGTATCGGTGAAGGCGGTCG
AGGCGTTCCTCGGCTGTTTCGCGGTCGAGGAGGTCGGCGATGCGGTCGCGCGCCGCGCCGCCGCGCTCCGCGCCGAGCGC
AAGGGGCTGACGCTCTGCGACGCGCTCGTGCTCGCGACCGCACAAATTAGCGGCCGCATTCTTGTTACACGCAATATCAA
GGTGTTTCCGGCAGCGATGCCGGGTATTCGCGTTCCCTACACGCTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT142541 WP_003026799.1 NZ_CP045782:c1735705-1735439 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT142541 NZ_CP060725:3675036-3675260 [Sphingopyxis sp. OPL5]
ATGCGCTTCCTCGCCGACATTCCCGACTCCGACGTCGAATGGCTCGATAGCCTCGCCGCCGGGCAAGGCGTCTCGCGCGC
CGAGCTGGTCCGCCGCGCGGTTGCGGCCTATCGCGCCGACGCCTCGGGCGACGCCATCGACAATGCGTTCGGCATCTGGC
GTGAGCGCGCCGATATCGGCGACGGGCTCAAATATCAGCGGCGCCTGCGGGGCAAGCGCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66