Detailed information of TA system TA142531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 596470..598058
• Replicon: chromosome
• Accession: NZ_CP045782
• Organism: Klebsiella pneumoniae strain LEMB18

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486JB51
• Locus tag: GFB99_RS03010
• Protein ID: WP_023316847.1
• Coordinates: 596736..598058 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: GFB99_RS03005
• Protein ID: WP_002904790.1
• Coordinates: 596470..596736 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFB99_RS02980	591614..592537	+	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-
GFB99_RS02985	592518..593672	-	1155	WP_048265552.1	FAD-dependent urate hydroxylase HpxO	-
GFB99_RS02990	594018..595397	+	1380	WP_004215088.1	purine permease	-
GFB99_RS02995	595375..595875	+	501	WP_060589175.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
GFB99_RS03000	595872..596198	+	327	WP_153676860.1	hydroxyisourate hydrolase	-
GFB99_RS03005	596470..596736	+	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GFB99_RS03010	596736..598058	+	1323	WP_023316847.1	type II toxin-antitoxin system HipA family toxin	Toxin
GFB99_RS03015	598075..599367	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
GFB99_RS03020	599509..600513	-	1005	WP_048337093.1	type 1 fimbrial protein	-
GFB99_RS03025	600501..603002	-	2502	WP_069334993.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49141.44 Da
• Isoelectric Point: 7.5357

>T142531 WP_023316847.1 NZ_CP045782:596736-598058 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T142531 NZ_CP060714:c5119902-5119576 [Diaphorobacter ruginosibacter]
ATGAATGCATGGACTGTCACGATCCATCCTGCGGCTTCGCCGGAGCTGTTGAGCCTGCCTGCAGACATGCAGGCCCGGTT
TCTGCATATCGCCGACATGCTGGAGGACTTCGGTCCTCAGCGTGTGGGCCTGCCACACGTTCGGCCATTGAGGCACAAGC
TGTGGGAAATGCGCCTGAATGGCCGGGATGGCATTGCGCGGGTCGTCTATGCAGCCGTCCAAGGTCGCCGTTTGCTGGTG
CTGCATGCGTTCGTGAAAAAGACGCAGGCCACACCACGCAGCGCCATCGAAACGGCTCTTTCGCGACTGGAGGCTCACGA
AGCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT142531 WP_002904790.1 NZ_CP045782:596470-596736 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT142531 NZ_CP060714:c5119579-5119292 [Diaphorobacter ruginosibacter]
ATGAAAACACTCACATCCTTGAAAGCGGAAATGCTGGCCCAGGACGACGTCCGCACGCACTATGAATCATTGGCCGATGA
ATTCGCCATGGCCCGCGAATTGATTGCAGCCCGCACGCGCGCAGGCCTCACGCAGGCGGATGTGGCGGCTCGCATGGGAA
CCACGCAAAGCGTCATCGCCAGAATCGAGGGAGGCAAGGCCACTCCCTCCCTGCGCAGCGTGCAGCGCTATGCCGAGGCC
ATAGGCTGCCGCGCGGTCGTGCGACTGGAAAGGCTGCCCCGGCGCTGA

Structures

Toxin

• AlphaFold DB: A0A486JB51

Antitoxin

• AlphaFold DB: A0A0H3GXD8