Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 70813..71455 Replicon chromosome
Accession NZ_CP045777
Organism Bacillus paranthracis strain CFSAN068816

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag GH772_RS01620 Protein ID WP_000635963.1
Coordinates 70813..71163 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag GH772_RS01625 Protein ID WP_000004570.1
Coordinates 71168..71455 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GH772_RS01605 67749..68207 - 459 WP_000344246.1 SprT family protein -
GH772_RS01610 68403..68519 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
GH772_RS01615 68577..70745 - 2169 WP_000450602.1 RNA-binding transcriptional accessory protein -
GH772_RS01620 70813..71163 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GH772_RS01625 71168..71455 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
GH772_RS01630 71757..72926 - 1170 WP_000390601.1 alanine racemase -
GH772_RS01635 73045..73995 - 951 WP_025991724.1 outer membrane lipoprotein carrier protein LolA -
GH772_RS01640 74152..74511 - 360 WP_000635037.1 holo-ACP synthase -
GH772_RS01645 74604..75176 + 573 WP_000906919.1 rhomboid family intramembrane serine protease -
GH772_RS01650 75169..76131 - 963 WP_000961151.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T142529 WP_000635963.1 NZ_CP045777:c71163-70813 [Bacillus paranthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T142529 NZ_CP060714:3079-4389 [Diaphorobacter ruginosibacter]
ATGAACGGTCGGTTTGTCGGCACCTGGAGCATAGAGGCCGGCGGCGACGTGTTGCAGTACGACGCCGAATGGGTGAATGA
CCCGGTCTTGCGCAGACCGTTGTCACTTTCGCTGCCCTTCACGCCTGGCAACCGTCCACACAAGGGCGACGCGGTACGGT
TCTATTTTCAGAATCTGCTGCCGGACAACGAGTACATCCTGGAACGTATCGCTCGCCGCTACAGGGTGCGTTCCATCGAC
GCCTATTCGCTGCTGAGCGAGATTGGGCGCGACTGCGTGGGTGCCCTGCAGATCTTGCCAGCAGGCGACATGCCGCCCGA
GGAACAGGGCGTGCACCGCGAGGCCCTGGACGAAGCCGAAATGGCCCGCCTCATCCGCGCGACGATCGACCCCGGGTTGT
TTGGAGACAGCGCCCGCGACGATGAAAAGTTTCGGATCTCGATTGCCGGCGCGCAGGAGAAAACCGCGTTCCTGCGCTGG
CAGGGTCAATGGTGCAGGCCGCTTGGAGCCACGCCGACGACGCATATCTTCAAGCTGCCGCTGGGCCTGATCGGCAACAT
GCAATTCGACATGCGGCATTCGGTGGAAAACGAATGGCTGTGTTCCGAACTGCTGGCCGCGTATGGACTGCCGGTGGCGC
GCTGCGAAGTGCTGCAATTCGAAGACCAGAAGGTCCTATCTGTGGAGCGCTTTGACAGGCGCGTGTGGAACGACAGCGAA
CTGCTCAGACTGCCGCAGGAGGACATGTGCCAAGCCATGGGCATGCCACCCATCCTCAAATACGAAAGCGACGGCGGTCC
CGGCGTGGATCGCATCATGGCGCTGCTGGACGGCTCCATGAATCGGGACCAGGACCGTCTCCAGTTCTTCACGGCCCAAC
TGCTGTTCTGGATGCTCTGTGCAACGGATGGCCACGCCAAGAATTTCAGCATCTTCCTGAGACCCGAGGGCCGGTTTGAG
CTCACGCCACTCTATGACGTGCTCTCGGCCTACCCGATTCTTGGGGCAGGTCCGGGAAAGCTCTCTCCACACAAGGCCCG
AATGGCCATGGCCGCGCGCTCCAAGAATGCCCACTGGCACATGAACAAGATCGTGCGTCGGCATTGGGTGGCGGTGGGGG
AGCGATATGGCATCGTTTCCCGGCAGGGCATGCCGGTGGATGCAGTGATCGATGAGATCGTGGAAATGACGCCCACCGTC
GTGCAACGGGTGCGTGGCAAGCTGCCGGAGGGGTTTCCTCAGGAGGTCGCGGAGGCAATATTCAACGGGTTGTTGGAGGC
GGCAAGGCGCCTGGGACGAAGCCGCGACTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT142529 WP_000004570.1 NZ_CP045777:c71455-71168 [Bacillus paranthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT142529 NZ_CP060714:2730-3011 [Diaphorobacter ruginosibacter]
ATGGACGAGTTCACTGTACGCACCACGGAGCAACTGCCGCAGTTGCTCAAGGCCTTCCGCAAGAACGCCAAGCTGACGCA
GGCGGATGTGGCGCTGCGCCTTGGCGTGACGCAGCAGACGGTCTCCGCAATGGAGCGCAATGCCGAAACCGTGAGCGCGG
AGCGCCTCATGAAGCTGATGGGTATTCTGGGCGTGGACTTGGTGTTGCGCGCCCGAACAAGCCAAGCGGCCCCACTCGCG
CAACCGGGCCACATGGTCAATGAGTCGCGTCCCCGCTGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References