Detailed information of TA system TA142518

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1845896..1846550
• Replicon: chromosome
• Accession: NZ_CP045771
• Organism: Citrobacter braakii strain MiY-A

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: GFC06_RS08660
• Protein ID: WP_016154348.1
• Coordinates: 1846143..1846550 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: GFC06_RS08655
• Protein ID: WP_016154349.1
• Coordinates: 1845896..1846162 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFC06_RS08630	1841108..1842541	-	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-
GFC06_RS08635	1842663..1843391	-	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
GFC06_RS08640	1843444..1843755	+	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
GFC06_RS08645	1843919..1844578	+	660	WP_153690042.1	hemolysin III family protein	-
GFC06_RS08650	1844658..1845638	-	981	WP_153690043.1	tRNA-modifying protein YgfZ	-
GFC06_RS08655	1845896..1846162	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
GFC06_RS08660	1846143..1846550	+	408	WP_016154348.1	protein YgfX	Toxin
GFC06_RS08665	1846651..1847172	-	522	WP_016154347.1	flavodoxin FldB	-
GFC06_RS08670	1847286..1848182	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
GFC06_RS08675	1848206..1848919	+	714	WP_153690044.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
GFC06_RS08680	1848925..1850658	+	1734	WP_016157413.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T142518 WP_016154348.1 NZ_CP045771:1846143-1846550 [Citrobacter braakii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T142518 NZ_CP060709:c3394732-3394418 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT142518 WP_016154349.1 NZ_CP045771:1845896-1846162 [Citrobacter braakii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT142518 NZ_CP060709:c3394421-3394005 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6