Detailed information of TA system TA142513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 291536..292112
• Replicon: chromosome
• Accession: NZ_CP045771
• Organism: Citrobacter braakii strain MiY-A

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: GFC06_RS01300
• Protein ID: WP_049282566.1
• Coordinates: 291825..292112 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A2I8S1U6
• Locus tag: GFC06_RS01295
• Protein ID: WP_016155614.1
• Coordinates: 291536..291838 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFC06_RS01280	288031..288225	+	195	WP_003830038.1	YbdD/YjiX family protein	-
GFC06_RS01285	288238..289194	+	957	WP_115191028.1	GTPase	-
GFC06_RS01290	289381..291228	+	1848	WP_153689754.1	NERD domain-containing protein/DEAD/DEAH box helicase	-
GFC06_RS01295	291536..291838	-	303	WP_016155614.1	BrnA antitoxin family protein	Antitoxin
GFC06_RS01300	291825..292112	-	288	WP_049282566.1	BrnT family toxin	Toxin
GFC06_RS01305	292346..293512	+	1167	WP_019078303.1	restriction endonuclease	-
GFC06_RS01310	293678..294001	+	324	Protein_256	endoribonuclease SymE	-
GFC06_RS01315	294096..294587	-	492	Protein_257	type VI secretion system tube protein Hcp	-
GFC06_RS01320	294588..295094	-	507	WP_049282567.1	hypothetical protein	-
GFC06_RS01325	295094..295456	-	363	WP_153690718.1	DUF2778 domain-containing protein	-
GFC06_RS01330	295731..296795	-	1065	WP_153689755.1	DUF2955 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11350.73 Da
• Isoelectric Point: 7.5237

>T142513 WP_049282566.1 NZ_CP045771:c292112-291825 [Citrobacter braakii]
MPMEFEWDANKAQSNHRKHGVRFEDAILVFDDPQHLSRQDRYENSEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHS

Nucleotide

• Length: 288 bp

>T142513 NZ_CP060709:2925167-2926240 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11466.12 Da
• Isoelectric Point: 10.1293

>AT142513 WP_016155614.1 NZ_CP045771:c291838-291536 [Citrobacter braakii]
MSIVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDKQWSEAIRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT142513 NZ_CP060709:2926233-2926604 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2I8S1U6