Detailed information of TA system TA142498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 3511751..3513009
• Replicon: chromosome
• Accession: NZ_CP045768
• Organism: Pseudomonas aeruginosa strain CFSAN084950

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GFD20_RS15945
• Protein ID: WP_023109087.1
• Coordinates: 3511751..3512506 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: GFD20_RS15950
• Protein ID: WP_003086329.1
• Coordinates: 3512641..3513009 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFD20_RS15935	3506923..3509466	+	2544	WP_153655544.1	acyl-homoserine-lactone acylase QuiP	-
GFD20_RS15940	3509622..3510983	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
GFD20_RS15945	3511751..3512506	-	756	WP_023109087.1	RES family NAD+ phosphorylase	Toxin
GFD20_RS15950	3512641..3513009	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
GFD20_RS15955	3513082..3514368	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
GFD20_RS15960	3514424..3516013	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
GFD20_RS15965	3516092..3516568	+	477	WP_153655545.1	transcription elongation factor GreAB	-
GFD20_RS29705	3516571..3516846	-	276	WP_176169208.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28264.40 Da
• Isoelectric Point: 7.7294

>T142498 WP_023109087.1 NZ_CP045768:c3512506-3511751 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T142498 NZ_CP060708:c4624622-4624281 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT142498 WP_003086329.1 NZ_CP045768:c3513009-3512641 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT142498 NZ_CP060708:4624617-4624693 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: V6AJU7