Detailed information of TA system TA142494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4366940..4367982
• Replicon: chromosome
• Accession: NZ_CP045767
• Organism: Pseudomonas sp. CFSAN084952

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GH769_RS19835
• Protein ID: WP_032903667.1
• Coordinates: 4366940..4367515 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A0W0MVK8
• Locus tag: GH769_RS19840
• Protein ID: WP_014719842.1
• Coordinates: 4367512..4367982 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GH769_RS19805	4362998..4363327	+	330	WP_020302001.1	hypothetical protein	-
GH769_RS19810	4363298..4363936	-	639	WP_020302002.1	LysE family transporter	-
GH769_RS19815	4363963..4364769	-	807	WP_032903670.1	2OG-Fe(II) oxygenase family protein	-
GH769_RS19820	4364995..4365687	+	693	WP_020302004.1	helix-turn-helix domain-containing protein	-
GH769_RS19825	4365696..4366331	-	636	WP_032903668.1	LysE family translocator	-
GH769_RS19830	4366488..4366859	+	372	WP_014719840.1	PH domain-containing protein	-
GH769_RS19835	4366940..4367515	-	576	WP_032903667.1	PIN domain-containing protein	Toxin
GH769_RS19840	4367512..4367982	-	471	WP_014719842.1	helix-turn-helix domain-containing protein	Antitoxin
GH769_RS19845	4368177..4369331	+	1155	WP_032903666.1	S-type pyocin domain-containing protein	-
GH769_RS19850	4369339..4369596	+	258	WP_032903665.1	colicin transporter	-
GH769_RS19855	4369655..4369939	+	285	WP_014719845.1	barstar family protein	-
GH769_RS19860	4369960..4370334	-	375	WP_019817492.1	cytochrome c family protein	-
GH769_RS19865	4370365..4370796	-	432	WP_032903664.1	VOC family protein	-
GH769_RS19870	4370961..4371323	+	363	WP_003194033.1	response regulator	-
GH769_RS19875	4371536..4372150	+	615	WP_032903663.1	biliverdin-producing heme oxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21405.84 Da
• Isoelectric Point: 4.7036

>T142494 WP_032903667.1 NZ_CP045767:c4367515-4366940 [Pseudomonas sp. CFSAN084952]
MRCSPFTAVYDACVLYPAALRDLLMWLGLSGLYRARWTGQIQDEWKRNVLKNRPDLTAAQVDCMSALMDIAIPDALVTGY
EDLCSSLDLPDPDDRHVLAAAIRSKAEVIVTFNLKDFPPHILAAYDIEPMHPDDFISDLWDLDQAAVLGAAQKQRRALKK
PAMEAGSYLEMLMRQGLVQTSKAMGPYEMML

Nucleotide

• Length: 576 bp

>T142494 NZ_CP060708:c3684820-3684713 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17496.17 Da
• Isoelectric Point: 6.1100

>AT142494 WP_014719842.1 NZ_CP045767:c4367982-4367512 [Pseudomonas sp. CFSAN084952]
MTINNLPKTILPLEKEVEAAVQGQRELASLLSTKFETQRIDIFDKEDKPHRLVLPTSALRLLVDILGELALGNAVKVVPV
HAELTSQEAADLLNVSRPHLVKMLEEGAIPFTKTGRHRRVRFSDLMAFKQRRDEQSQEAMEALVQQAQELGMGYDG

Nucleotide

• Length: 471 bp

>AT142494 NZ_CP060708:3684869-3684935 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Structures

Antitoxin

• AlphaFold DB: A0A0W0MVK8