Detailed information of TA system TA142468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 41982..42559
• Replicon: plasmid pCFSAN008100
• Accession: NZ_CP045750
• Organism: Listeria monocytogenes strain CFSAN008100

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: GH756_RS15625
• Protein ID: WP_003728465.1
• Coordinates: 42218..42559 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: GH756_RS15620
• Protein ID: WP_003728464.1
• Coordinates: 41982..42224 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GH756_RS15575	37254..37970	-	717	WP_003731678.1	AP2 domain-containing protein	-
GH756_RS15580	37986..38708	-	723	WP_003728517.1	CRISPR-associated protein Cas5	-
GH756_RS15585	38712..39137	-	426	WP_003728518.1	hypothetical protein	-
GH756_RS15590	39138..39386	-	249	WP_003728519.1	hypothetical protein	-
GH756_RS15595	39508..39996	-	489	WP_003728520.1	hypothetical protein	-
GH756_RS15600	40155..40532	-	378	WP_003769263.1	DUF3850 domain-containing protein	-
GH756_RS15605	40551..41213	-	663	WP_003731676.1	DUF2786 domain-containing protein	-
GH756_RS15610	41213..41539	-	327	WP_003728462.1	hypothetical protein	-
GH756_RS15615	41670..41921	+	252	WP_003728463.1	hypothetical protein	-
GH756_RS15620	41982..42224	+	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GH756_RS15625	42218..42559	+	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GH756_RS15630	42752..44887	-	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
GH756_RS15635	44887..45246	-	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
GH756_RS15640	45526..46080	+	555	WP_003728468.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..51115	51115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 14629.86 Da
• Isoelectric Point: 12.8531

>T142468 WP_003728465.1 NZ_CP045750:42218-42559 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 342 bp

>T142468 NZ_CP060704:5459688-5459978 [Chitinimonas koreensis]
ATGGTCGAGATACGTCAAACGGACGGTTACGCCCACTGGTTCGCAGGACTGCGGGATCGCACGGCACGCGCCCGGATCGA
CATCCGCATCCGCCGCCTGTCGCTCGACAACCCCGGCGACGTCAAACCCGTAGGCGGCGGCGTATCCGAGCTTCGCATCG
ACCGTGGGCCGGGCTATCGCATCTACTTCGTCCGGCGCGGCGACACGCTCGTCGTCCTGCTGGCCGGCGGCGGCAAGTCC
ACGCAGGAACAGGACATCCGGAACGCGCTCGAACTGGCCCGTTCGATCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 10400.12 Da
• Isoelectric Point: 12.7030

>AT142468 WP_003728464.1 NZ_CP045750:41982-42224 [Listeria monocytogenes]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
K

Nucleotide

• Length: 243 bp

>AT142468 NZ_CP060704:5459989-5460294 [Chitinimonas koreensis]
ATGGCAGACACTGCAACCCGCGCCTGGGACCCGGCGGAACACCTCGATAGCGAAGAAGATATGGCGGCCTATCTCGAGGC
CGCCCTCGAAGACGGCGACCCGGCCCTGGTCACCGCCGCCTTGGGCGATATCGCCCGCGCCAAGGGCATGACCCGCATCG
CGCGCGAGGCCGGTCTGAGCCGCGAAAGCCTGTACAAGGCGCTTTCGCCCAACGGCAACCCGGAGCTGGCGACGATCCTG
AAGGTCGTCTCCGTGCTCGGCCTGCGGCTGCACGCTACCCCCGCACCGGCGAAGCTACCGGGATAG

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5