Detailed information of TA system TA142467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 17011..17588
• Replicon: plasmid pCFSAN044836
• Accession: NZ_CP045744
• Organism: Listeria innocua strain CFSAN044836

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: GH761_RS14580
• Protein ID: WP_003728465.1
• Coordinates: 17247..17588 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: GH761_RS14575
• Protein ID: WP_003728464.1
• Coordinates: 17011..17253 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GH761_RS14530	12283..12999	-	717	WP_003731678.1	AP2 domain-containing protein	-
GH761_RS14535	13015..13737	-	723	WP_003728517.1	CRISPR-associated protein Cas5	-
GH761_RS14540	13741..14166	-	426	WP_003728518.1	hypothetical protein	-
GH761_RS14545	14167..14415	-	249	WP_003728519.1	hypothetical protein	-
GH761_RS14550	14537..15025	-	489	WP_003728520.1	hypothetical protein	-
GH761_RS14555	15184..15561	-	378	WP_003769263.1	DUF3850 domain-containing protein	-
GH761_RS14560	15580..16242	-	663	WP_003731676.1	DUF2786 domain-containing protein	-
GH761_RS14565	16242..16568	-	327	WP_003728462.1	hypothetical protein	-
GH761_RS14570	16699..16950	+	252	WP_003728463.1	hypothetical protein	-
GH761_RS14575	17011..17253	+	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GH761_RS14580	17247..17588	+	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GH761_RS14585	17781..19916	-	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
GH761_RS14590	19916..20275	-	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
GH761_RS14595	20555..21109	+	555	WP_045554658.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ClpL	-	1..57553	57553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12827.74 Da
• Isoelectric Point: 9.2830

>T142467 WP_003728465.1 NZ_CP045744:17247-17588 [Listeria innocua]
MVKQGDIIKINLNPKQGHEQQGYRPYICLSHHLVSDYANIAIFAPISNTPRTYPLYVPLTGTTTTGKVLLDQLVTIDYNA
RKHQYVETVSDPLLTKLLDTVKVIFQKNERSTN

Nucleotide

• Length: 342 bp

>T142467 NZ_CP060704:c4822935-4822645 [Chitinimonas koreensis]
ATGCCACGGCTGATCTGGTCGCCGCAGGCGCTGCACGATGTGCAGCGCCTTTATCGTTTCCTGGCGGCGAACAATGCCGA
TGCGGCCCGCCGCGCCGTCAAGGCGATCCGCGACGGCGTCAAACTGCTCGCCTCGCAGCCCGGCGTCGGCCGCCCGGCCG
AGGAGATGGAACCCGAATACCGCGAGTGGCTGATCGACTTCGGCGACAGCGGCTACATCGCGCTGTACCGCTACGACGGC
AAAACCGCGCTGATCCTGGCGGTACGCCATCAGCGCGAAGCCGGCTACTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9207.48 Da
• Isoelectric Point: 4.3867

>AT142467 WP_003728464.1 NZ_CP045744:17011-17253 [Listeria innocua]
MTTVSVKKWGNSKAVRLPNNLLNALDIKENDDLKVEVINNQMILTKTAIDLRIEDLFANYQGEPFQTELQTFEPIGDEKW

Nucleotide

• Length: 243 bp

>AT142467 NZ_CP060704:c4823189-4822923 [Chitinimonas koreensis]
GTGGCGATCAAGATCGATCCCGAGACCCGGGAGCGCATCAAGCGTCTGGCGGCTGCGCGCGACCGCACGCCGCACTGGAT
CATGCGCGAGGCGATCAGCCAGTACGTCGAGCGCGAGGAGAAGCGCGAAGCGTTGCGGCAGGACGGCATCATGGCCTGGA
ACGCGTACCACGCCAACGGGCTGCATGCCACCGCCGACGAGGCCGATGCCTGGCTCGCCGAGCTGGAAGCCGGCCGCGAC
GTCGAGCCGCCTGAATGCCACGGCTGA

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5