Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 157864..158507 Replicon plasmid pTHNK130-1
Accession NZ_CP045742
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag GHR40_RS23060 Protein ID WP_001044768.1
Coordinates 157864..158280 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag GHR40_RS23065 Protein ID WP_001261287.1
Coordinates 158277..158507 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHR40_RS23045 154363..154953 - 591 WP_000194575.1 hypothetical protein -
GHR40_RS23050 154953..155210 - 258 WP_000343085.1 hypothetical protein -
GHR40_RS23055 155564..157702 + 2139 WP_000350638.1 AAA family ATPase -
GHR40_RS23060 157864..158280 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
GHR40_RS23065 158277..158507 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GHR40_RS23070 159068..159382 + 315 WP_001302628.1 hypothetical protein -
GHR40_RS23075 159435..160214 + 780 WP_000535297.1 hypothetical protein -
GHR40_RS23080 160265..161044 + 780 WP_001302707.1 site-specific integrase -
GHR40_RS23085 161182..161457 - 276 WP_000239529.1 hypothetical protein -
GHR40_RS23090 161451..162095 - 645 WP_000633913.1 AAA family ATPase -
GHR40_RS23095 162324..163295 + 972 WP_001103690.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-14 / fosA3 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-IIa / aadA5 - 1..180783 180783


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T142465 WP_001044768.1 NZ_CP045742:c158280-157864 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T142465 NZ_CP060704:3560953-3561135 [Chitinimonas koreensis]
ATGGACAGCAAGCAAATCATCAAGCAGCTTGAGGCGGATGGCTGGTTCCTGGCCCGAGTGAAGGGCTCGCATCACCAGTT
CAAGCATCCGACCAAGCCTGGCCTGGTGACGGTCAAGCATCCTGACGGCGACATTCCCGCCGGCACGCTGAACTCGATCC
GCAAGCAAGCGGGATGGAAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT142465 WP_001261287.1 NZ_CP045742:c158507-158277 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT142465 NZ_CP060704:3561147-3561539 [Chitinimonas koreensis]
ATGCGTTTTCCCGTAGTACTGCATACCGATGACGGCGTCCGCTACGGCGTGACGGTGCCCGACCTTCCCGGCTGTTTTTC
GGCCGGCGATACCTTCGACGAGGCGCTCGACAGCGTGGCCGAAGCGATCGACCTCCACCTCGAGGGCCTGGTCGAAGAAG
GCAGCGAGGTGCCGGTAGCCGGCGCGATCGCCGAGCATCGCGCCAACCCCGAATACGCCGCCGGCGTGTGGGCCGTCGTC
GAGGTGGACGTGACCCGCTTCGACGGCAAGGCCGAGAAGATCAACATCACCTTGCCGCGCCGCTTGCTTGCGCGGATCGA
CGAATACGCCCGTGCCCACGGGTCGACGCGGTCGGGGTTTCTCGCAGATGCCGCGCGGGCGGCGATGCGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References