Detailed information of TA system TA142459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4488258..4488876
• Replicon: chromosome
• Accession: NZ_CP045741
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: GHR40_RS21650
• Protein ID: WP_001290581.1
• Coordinates: 4488658..4488876 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: GHR40_RS21645
• Protein ID: WP_000344800.1
• Coordinates: 4488258..4488632 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHR40_RS21635	4483347..4484540	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
GHR40_RS21640	4484563..4487712	+	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
GHR40_RS21645	4488258..4488632	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
GHR40_RS21650	4488658..4488876	+	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
GHR40_RS21655	4489048..4489599	+	552	WP_000102564.1	maltose O-acetyltransferase	-
GHR40_RS21660	4489715..4490185	+	471	WP_000136192.1	YlaC family protein	-
GHR40_RS21665	4490349..4491899	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
GHR40_RS21670	4491941..4492294	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
GHR40_RS21680	4492673..4492984	+	312	WP_000409911.1	MGMT family protein	-
GHR40_RS21685	4493015..4493587	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T142459 WP_001290581.1 NZ_CP045741:4488658-4488876 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T142459 NZ_CP060703:c4858891-4858544 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT142459 WP_000344800.1 NZ_CP045741:4488258-4488632 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT142459 NZ_CP060703:c4859152-4858901 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5