Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1892720..1893374 Replicon chromosome
Accession NZ_CP045741
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GHR40_RS09045 Protein ID WP_000244777.1
Coordinates 1892967..1893374 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GHR40_RS09040 Protein ID WP_000354046.1
Coordinates 1892720..1892986 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHR40_RS09015 1887889..1888632 + 744 WP_000951964.1 SDR family oxidoreductase -
GHR40_RS09020 1888689..1890122 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
GHR40_RS09025 1890167..1890478 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
GHR40_RS09030 1890642..1891301 + 660 WP_000250274.1 hemolysin III family protein -
GHR40_RS09035 1891497..1892477 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GHR40_RS09040 1892720..1892986 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GHR40_RS09045 1892967..1893374 + 408 WP_000244777.1 toxin CptA Toxin
GHR40_RS09050 1893414..1893935 - 522 WP_001055874.1 flavodoxin FldB -
GHR40_RS09055 1894047..1894943 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GHR40_RS09060 1894968..1895678 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GHR40_RS09065 1895684..1897417 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T142442 WP_000244777.1 NZ_CP045741:1892967-1893374 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T142442 NZ_CP060698:321347-321646 [Amniculibacterium sp. G2-70]
ATGTACTTTATTGAGAAAACGGCTGAGTTTGATAAATGGCTTAGAAAACTAAAAGATACCCGAGCGAAAGCTAAAGTTTT
ATTTAGGATTCAGAAGTTAGAAAAAGACGAACATTTTGGTGATTATGAACCAGTTGGCAATGGAATACGAGAGCTAAAAA
TCAACTATGCAAAAGGATATAGAATTTACTTTAAAGAGACCGATGGGAAAATTATCATTCTACTCATTGGTGGAGACAAG
TCTTCTCAACAAAAAGATATTGAAAAGGCAAAAGACATTTGGAATAAAATAAAAAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT142442 WP_000354046.1 NZ_CP045741:1892720-1892986 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT142442 NZ_CP060698:321650-321934 [Amniculibacterium sp. G2-70]
ATGGAAACATCAAAATTTGATATTGCAGATTATTTGGACAGCAACGAAATGATTGCTGAATATCTTAATGAGGTTTTAGA
AAACGGTTATGATTCTGAAATTATCACAGCAATCGGAAACATTGCAAAAGCAATTGGAATGACAAAAATTGCTGAAGAAA
CAGGACTTAGCAGACCAAGTTTGTACAAAGCATTATCTGAAGGTGCTAAACCTCAATTTTCAACAATAATGAAAGTACTG
AGAGCTGTCGGAGGACAAATACAAATCAACCCAGCACATATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References