Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 581146..581643 Replicon chromosome
Accession NZ_CP045741
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GHR40_RS02835 Protein ID WP_001173343.1
Coordinates 581146..581319 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GHR40_RS02840 Protein ID WP_000398619.1
Coordinates 581347..581643 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHR40_RS02825 577816..579273 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
GHR40_RS02830 579510..581027 + 1518 WP_001295074.1 lysine--tRNA ligase -
GHR40_RS02835 581146..581319 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GHR40_RS02840 581347..581643 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GHR40_RS02845 581870..582142 - 273 WP_000405651.1 N-acetyltransferase -
GHR40_RS02850 582154..582384 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
GHR40_RS02855 582565..584196 + 1632 WP_001216456.1 sensor histidine kinase -
GHR40_RS02860 584193..584912 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T142436 WP_001173343.1 NZ_CP045741:c581319-581146 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T142436 NZ_CP060693:386211-386489 [Aliarcobacter cryaerophilus]
ATGAGTAAATTAGCAACATTTGATATATCACAATATCTTGATAATAAAGAGATGATAGCAGAATACTTATCACAAGTGAT
AGAAGATGGTGATATGGATGAATTATTAGAAGCTATAGGAAATATAGCAAAAGCTAAAGGAATAAGCCAAATAGCAGCTG
ATACAGGATTAGGGAGAGAAAGTTTATATAAAACATTTCAATCAGGTTCAAAGCCTAGATTTGAAACTGTGATGAAAGTA
CTTCGTAGTTTAGGTGTAAATATCAAAGCTATTGCATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT142436 WP_000398619.1 NZ_CP045741:c581643-581347 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT142436 NZ_CP060693:385919-386218 [Aliarcobacter cryaerophilus]
ATGTATTTAATAAAACAAACAGAACTATTCTCAAAATGGTTATATAAATTAAAAGATATACAAGGTAAAGTTTCTATATT
ACGAAGAGTTGATCGTATGAAAAAAGGAAATTTTGGCGATTTTAAATCCATAGGTGATGGTATATGTGAACTTAGGTTTA
CAATTGGTCCAGGATATAGAGTTTACTATACTCAAAAAGGTGATGAAATTATTATTTTATTAGTTGGTGGAGATAAATCA
ACCCAATCAAAAGATATAGAAAAAGCAAAAGAACTTTTAAAGGAATTTGATAATGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References