Detailed information of TA system TA142434

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 399409..400223
• Replicon: chromosome
• Accession: NZ_CP045741
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: GHR40_RS01925
• Protein ID: WP_001054376.1
• Coordinates: 399409..399666 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: GHR40_RS01930
• Protein ID: WP_001309181.1
• Coordinates: 399678..400223 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHR40_RS01905	394697..395803	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
GHR40_RS01910	395868..396848	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
GHR40_RS01915	397431..398671	-	1241	Protein_374	DNA helicase	-
GHR40_RS01920	398787..399032	+	246	Protein_375	GNAT family N-acetyltransferase	-
GHR40_RS01925	399409..399666	+	258	WP_001054376.1	hypothetical protein	Toxin
GHR40_RS01930	399678..400223	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
GHR40_RS01935	400279..401025	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
GHR40_RS01940	401194..401412	+	219	Protein_379	hypothetical protein	-
GHR40_RS23375	401450..401566	+	117	Protein_380	VOC family protein	-
GHR40_RS01945	401811..402932	+	1122	WP_000010829.1	M42 family metallopeptidase	-
GHR40_RS01950	402929..403207	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
GHR40_RS01955	403219..404532	+	1314	WP_000460842.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	387657..408447	20790

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T142434 WP_001054376.1 NZ_CP045741:399409-399666 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T142434 NZ_CP060692:1133512-1133778 [Aliarcobacter cryaerophilus]
ATGGTAAACTATAAACTTGTTTACACTAAAAAAGCTGGAAAAGATGCCAAGAAATTATCTAATTCAGGCTTAAAATCAAA
GGCTTTGGAATTATTAGAGATAATTTCTTTAAATCCATATCAAAATCCCCCGCCTTATGAAAAACTAATTGGTGATTTAA
ATAGTGCAATATCAAGAAGAATAAATATTCAACACAGATTAGTTTATGAAGTTTTAGAAGATATTAAAACTATAAAAGTG
ATACGAATGTGGTCACATTATGAGTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT142434 WP_001309181.1 NZ_CP045741:399678-400223 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT142434 NZ_CP060692:1133273-1133518 [Aliarcobacter cryaerophilus]
ATGACGAAAGTTATGACAGTTAGTCAAGTTCGAGCAGATATTTATAATATTATTGATGAAACAGCATTAACACATGAACC
AATACTTATAACTGGTAAAAGAACAAATGCTGTTATGTTATCTCAAGAAGATTGGAATGCCATCCAAGAGACTTTATATC
TAACATCAATTCCAAATATGAGAGAATCTATAATTAAAGGATTAAATACATCTATTGAAGATTGTGATGAAGAGTTAGAA
TGGTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8