Detailed information of TA system TA142429

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 25501..26180
• Replicon: chromosome
• Accession: NZ_CP045741
• Organism: Escherichia coli strain DH5alpha

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: GHR40_RS00180
• Protein ID: WP_000854672.1
• Coordinates: 25839..26180 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: GHR40_RS00175
• Protein ID: WP_000070395.1
• Coordinates: 25501..25818 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHR40_RS00125	20548..21411	+	864	WP_001065553.1	GTPase family protein	-
GHR40_RS00130	21503..22324	+	822	WP_000197389.1	DUF945 domain-containing protein	-
GHR40_RS00135	22541..22732	+	192	Protein_25	DeoR family transcriptional regulator	-
GHR40_RS00140	22728..22955	+	228	WP_001548158.1	hypothetical protein	-
GHR40_RS00145	22955..23398	+	444	WP_000824223.1	hypothetical protein	-
GHR40_RS00150	23421..23888	+	468	WP_001547765.1	hypothetical protein	-
GHR40_RS00155	23965..24204	+	240	WP_000194654.1	DUF905 domain-containing protein	-
GHR40_RS00160	24302..24760	+	459	WP_000211838.1	antirestriction protein	-
GHR40_RS00165	24776..25252	+	477	WP_000811693.1	RadC family protein	-
GHR40_RS00170	25261..25482	+	222	WP_000691994.1	DUF987 domain-containing protein	-
GHR40_RS00175	25501..25818	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
GHR40_RS00180	25839..26180	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
GHR40_RS00190	26752..28005	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
GHR40_RS00195	28017..29120	-	1104	WP_001285288.1	glutamate 5-kinase	-
GHR40_RS00200	29408..29767	+	360	Protein_37	porin	-
GHR40_RS00205	29874..31082	+	1209	WP_001352368.1	IS4-like element ISVsa5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T142429 WP_000854672.1 NZ_CP045741:25839-26180 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T142429 NZ_CP060678:c4515071-4514796 [Salmonella enterica]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT142429 WP_000070395.1 NZ_CP045741:25501-25818 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT142429 NZ_CP060678:c4515334-4515074 [Salmonella enterica]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684