Detailed information of TA system TA142421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 1201814..1203235
• Replicon: chromosome
• Accession: NZ_CP045739
• Organism: Pseudomonas aeruginosa strain AG1

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: PaeAG1_RS05835
• Protein ID: WP_025297643.1
• Coordinates: 1202162..1203235 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: PaeAG1_RS05830
• Protein ID: WP_158025315.1
• Coordinates: 1201814..1202107 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PaeAG1_RS05785	1196917..1197129	+	213	WP_023128602.1	hypothetical protein	-
PaeAG1_RS05790	1197220..1197675	-	456	WP_025297646.1	helix-turn-helix domain-containing protein	-
PaeAG1_RS05795	1197840..1198235	+	396	WP_023128603.1	hypothetical protein	-
PaeAG1_RS05800	1198278..1198748	+	471	WP_023128604.1	hypothetical protein	-
PaeAG1_RS05805	1198751..1199134	+	384	WP_025297645.1	hypothetical protein	-
PaeAG1_RS05810	1199216..1199794	+	579	WP_025297644.1	hypothetical protein	-
PaeAG1_RS05815	1200125..1200489	+	365	Protein_1150	DNA binding protein	-
PaeAG1_RS05820	1200713..1200907	-	195	WP_023128607.1	OprD family outer membrane porin	-
PaeAG1_RS05825	1200882..1201675	+	794	Protein_1152	transporter substrate-binding domain-containing protein	-
PaeAG1_RS05830	1201814..1202107	-	294	WP_158025315.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
PaeAG1_RS05835	1202162..1203235	-	1074	WP_025297643.1	type II toxin-antitoxin system RnlA family toxin	Toxin
PaeAG1_RS05840	1203950..1204204	+	255	WP_023128610.1	hypothetical protein	-
PaeAG1_RS05845	1204502..1204798	+	297	WP_023128612.1	hypothetical protein	-
PaeAG1_RS05850	1204851..1206434	-	1584	WP_025297642.1	hypothetical protein	-
PaeAG1_RS05855	1206714..1207142	+	429	WP_025297641.1	S24 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1188544..1261091	72547

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal (6-87)

Antitoxin

• Antitoxin RnlB/LsoB (2-96)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40381.89 Da
• Isoelectric Point: 6.1830

>T142421 WP_025297643.1 NZ_CP045739:c1203235-1202162 [Pseudomonas aeruginosa]
VTERDYKNINLKRCIIEETTHCFIRDNGLKLRSYGDKPGSSGLRVVFGKAGIEDATLDIFFTNVGASTLTYKVGKNQALG
QQLADALYETIHPDEFVTMNLGIKGVDLDDAEALIKELTESANADIEVASRTSADRKHIWKLKSKKNLDELTVTQHENTH
KLQIQGRPLSCYQQLSYLLTELLEINALECILFKKEENRSEFVCAEVAESVLKERFGDCYANLPQTSRRLLLASLCVRLA
SPALPDYCMLVYPELRTLEGAIKQKLAEKNLSNQEESFGGFFTKTNGQFYLKQQYHADVGDAKLQGHLDNAYTFFNKQRH
GLFHMEEMPSVSRMISNITQAVSLCDEAYDHIKNLYS

Nucleotide

• Length: 1074 bp

>T142421 NZ_CP060678:885477-885764 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11039.38 Da
• Isoelectric Point: 4.5163

>AT142421 WP_158025315.1 NZ_CP045739:c1202107-1201814 [Pseudomonas aeruginosa]
MTVVTATSYENPLSRLSIIASEMRNTSHSSKEIVLFDLLCSNGEEWNRFVSINYNGTDFEKSTCSIVSKSDIPTDLLETQ
TRFFQIHPQYLLDSVLN

Nucleotide

• Length: 294 bp

>AT142421 NZ_CP060678:885751-886053 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG