Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4744212..4744828 Replicon chromosome
Accession NZ_CP045726
Organism Citrobacter freundii strain zone4

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag GHT30_RS22560 Protein ID WP_003028682.1
Coordinates 4744212..4744586 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag GHT30_RS22565 Protein ID WP_043018956.1
Coordinates 4744586..4744828 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHT30_RS22545 4741715..4742617 + 903 WP_003847898.1 formate dehydrogenase O subunit beta -
GHT30_RS22550 4742614..4743249 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
GHT30_RS22555 4743246..4744175 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
GHT30_RS22560 4744212..4744586 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
GHT30_RS22565 4744586..4744828 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
GHT30_RS22570 4745033..4745941 + 909 WP_205688231.1 alpha/beta hydrolase fold domain-containing protein -
GHT30_RS22575 4746092..4747033 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
GHT30_RS22580 4747078..4747515 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
GHT30_RS22585 4747512..4748381 - 870 WP_136397728.1 virulence factor BrkB family protein -
GHT30_RS22590 4748375..4748974 - 600 WP_046671351.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T142414 WP_003028682.1 NZ_CP045726:c4744586-4744212 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T142414 NZ_CP060677:17701-18006 [Salmonella enterica]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT142414 WP_043018956.1 NZ_CP045726:c4744828-4744586 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT142414 NZ_CP060677:17481-17699 [Salmonella enterica]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References