Detailed information of TA system TA142413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4560957..4561623
• Replicon: chromosome
• Accession: NZ_CP045726
• Organism: Citrobacter freundii strain zone4

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: GHT30_RS21675
• Protein ID: WP_134214823.1
• Coordinates: 4561264..4561623 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: GHT30_RS21670
• Protein ID: WP_134214821.1
• Coordinates: 4560957..4561274 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT30_RS21630	4556245..4557069	+	825	WP_048216971.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
GHT30_RS21635	4557137..4558360	+	1224	WP_003031639.1	L-sorbose 1-phosphate reductase	-
GHT30_RS21640	4558362..4559174	+	813	WP_205688220.1	shikimate 5-dehydrogenase	-
GHT30_RS21645	4559208..4559525	-	318	WP_054527950.1	CcdB family protein	-
GHT30_RS21650	4559525..4559818	-	294	WP_003844689.1	type II toxin-antitoxin system CcdA family antitoxin	-
GHT30_RS21655	4559915..4560280	-	366	WP_134214819.1	hypothetical protein	-
GHT30_RS21660	4560412..4560585	+	174	WP_032938222.1	hypothetical protein	-
GHT30_RS21665	4560656..4560817	+	162	WP_003841416.1	phage protein	-
GHT30_RS21670	4560957..4561274	-	318	WP_134214821.1	helix-turn-helix domain-containing protein	Antitoxin
GHT30_RS21675	4561264..4561623	-	360	WP_134214823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHT30_RS21680	4561837..4562526	+	690	WP_003841421.1	dipeptidase PepE	-
GHT30_RS21685	4562673..4564304	-	1632	WP_003031618.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-87)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13456.53 Da
• Isoelectric Point: 10.2555

>T142413 WP_134214823.1 NZ_CP045726:c4561623-4561264 [Citrobacter freundii]
MTKPLYWVGQARKDLLAMPEHVRDTFGFAFWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGSAWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVI

Nucleotide

• Length: 360 bp

>T142413 NZ_CP060676:c3919765-3919238 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11504.44 Da
• Isoelectric Point: 8.9375

>AT142413 WP_134214821.1 NZ_CP045726:c4561274-4560957 [Citrobacter freundii]
MSYEVEASSGNVYVDLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT142413 NZ_CP060676:c3920052-3919762 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA