Detailed information of TA system TA142409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2406725..2407364
• Replicon: chromosome
• Accession: NZ_CP045726
• Organism: Citrobacter freundii strain zone4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: GHT30_RS11530
• Protein ID: WP_003020221.1
• Coordinates: 2406725..2406901 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GHT30_RS11535
• Protein ID: WP_123924900.1
• Coordinates: 2406948..2407364 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT30_RS11510	2403503..2403733	-	231	WP_048217543.1	DUF2554 family protein	-
GHT30_RS11515	2403923..2404048	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
GHT30_RS11520	2404048..2405058	-	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
GHT30_RS11525	2405058..2406461	-	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
GHT30_RS11530	2406725..2406901	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
GHT30_RS11535	2406948..2407364	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GHT30_RS11540	2407457..2408866	+	1410	WP_046670716.1	PLP-dependent aminotransferase family protein	-
GHT30_RS11545	2409195..2410340	+	1146	WP_003020212.1	ABC transporter substrate-binding protein	-
GHT30_RS11550	2410357..2411373	+	1017	WP_003020210.1	ABC transporter ATP-binding protein	-
GHT30_RS11555	2411374..2412318	+	945	WP_003843727.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T142409 WP_003020221.1 NZ_CP045726:2406725-2406901 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T142409 NZ_CP060676:c3238305-3237994 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACACTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGCACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATGATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT142409 WP_123924900.1 NZ_CP045726:2406948-2407364 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT142409 NZ_CP060676:c3237997-3237551 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A8B5QF36