Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 927373..928027 Replicon chromosome
Accession NZ_CP045726
Organism Citrobacter freundii strain zone4

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag GHT30_RS04600 Protein ID WP_003026936.1
Coordinates 927620..928027 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag GHT30_RS04595 Protein ID WP_003026938.1
Coordinates 927373..927639 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHT30_RS04570 922574..924007 - 1434 WP_044700805.1 6-phospho-beta-glucosidase BglA -
GHT30_RS04575 924128..924856 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
GHT30_RS04580 924909..925220 + 312 WP_044700802.1 N(4)-acetylcytidine aminohydrolase -
GHT30_RS04585 925383..926042 + 660 WP_003026947.1 hemolysin III family protein -
GHT30_RS04590 926136..927116 - 981 WP_119174530.1 tRNA-modifying protein YgfZ -
GHT30_RS04595 927373..927639 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
GHT30_RS04600 927620..928027 + 408 WP_003026936.1 protein YgfX Toxin
GHT30_RS04605 928072..928593 - 522 WP_003026933.1 flavodoxin FldB -
GHT30_RS04610 928707..929603 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
GHT30_RS04615 929627..930340 + 714 WP_003825520.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GHT30_RS04620 930346..932079 + 1734 WP_016150943.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T142400 WP_003026936.1 NZ_CP045726:927620-928027 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T142400 NZ_CP060676:1662091-1662309 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT142400 WP_003026938.1 NZ_CP045726:927373-927639 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT142400 NZ_CP060676:1661689-1662063 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References