Detailed information of TA system TA142399

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 769527..770280
• Replicon: chromosome
• Accession: NZ_CP045726
• Organism: Citrobacter freundii strain zone4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: GHT30_RS03770
• Protein ID: WP_016154457.1
• Coordinates: 769527..769904 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GHT30_RS03775
• Protein ID: WP_162838704.1
• Coordinates: 769954..770280 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT30_RS03745	765671..767530	+	1860	WP_003838200.1	bifunctional glutathionylspermidine amidase/synthase	-
GHT30_RS03750	767569..767868	-	300	WP_032950098.1	hypothetical protein	-
GHT30_RS03755	767880..768725	-	846	Protein_736	DUF4942 domain-containing protein	-
GHT30_RS03760	768806..769009	-	204	WP_009652505.1	DUF957 domain-containing protein	-
GHT30_RS03765	769039..769530	-	492	WP_009652438.1	hypothetical protein	-
GHT30_RS03770	769527..769904	-	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
GHT30_RS03775	769954..770280	-	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GHT30_RS03780	770337..770558	-	222	WP_009652392.1	DUF987 domain-containing protein	-
GHT30_RS03785	770572..771054	-	483	WP_016154455.1	DNA repair protein RadC	-
GHT30_RS03790	771066..771284	-	219	WP_009652503.1	hypothetical protein	-
GHT30_RS03795	771295..771756	-	462	WP_045890390.1	antirestriction protein	-
GHT30_RS03800	771785..772039	-	255	WP_009652519.1	hypothetical protein	-
GHT30_RS03805	772039..772857	-	819	WP_009652424.1	DUF945 domain-containing protein	-
GHT30_RS03810	772961..773194	-	234	WP_009652474.1	DUF905 domain-containing protein	-
GHT30_RS03815	773250..773471	-	222	WP_009652443.1	hypothetical protein	-
GHT30_RS03820	773554..774066	-	513	WP_009652395.1	DUF4234 domain-containing protein	-
GHT30_RS03825	774115..774585	-	471	WP_009652431.1	hypothetical protein	-
GHT30_RS03830	774632..774943	-	312	WP_009652410.1	hypothetical protein	-
GHT30_RS03835	774956..775222	-	267	WP_009652486.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T142399 WP_016154457.1 NZ_CP045726:c769904-769527 [Citrobacter freundii]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T142399 NZ_CP060676:c942811-942708 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT142399 WP_162838704.1 NZ_CP045726:c770280-769954 [Citrobacter freundii]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT142399 NZ_CP060676:942706-942851 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Structures

Toxin

• AlphaFold DB: R8WN26