Detailed information of TA system TA142397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 250232..250863
• Replicon: chromosome
• Accession: NZ_CP045726
• Organism: Citrobacter freundii strain zone4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0J1MPQ6
• Locus tag: GHT30_RS01160
• Protein ID: WP_003837806.1
• Coordinates: 250232..250507 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GHT30_RS01165
• Protein ID: WP_205688252.1
• Coordinates: 250504..250863 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT30_RS01150	246283..249030	+	2748	WP_172750167.1	ribosome-associated ATPase/putative transporter RbbA	-
GHT30_RS01155	249030..250154	+	1125	WP_071687967.1	ABC transporter permease	-
GHT30_RS01160	250232..250507	+	276	WP_003837806.1	type II toxin-antitoxin system HicA family toxin	Toxin
GHT30_RS01165	250504..250863	+	360	WP_205688252.1	toxin-antitoxin system HicB family antitoxin	Antitoxin
GHT30_RS01170	250976..251377	-	402	WP_032937232.1	nickel-responsive transcriptional regulator NikR	-
GHT30_RS01175	251427..252005	-	579	WP_003837810.1	4'-phosphopantetheinyl transferase AcpT	-
GHT30_RS01180	252068..253117	-	1050	WP_003023393.1	AI-2E family transporter	-
GHT30_RS01185	253251..254468	+	1218	WP_205688253.1	MFS transporter	-
GHT30_RS01190	254472..255029	-	558	WP_003837816.1	DcrB family lipoprotein	-
GHT30_RS01195	255102..255767	-	666	WP_003837818.1	7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10210.88 Da
• Isoelectric Point: 10.7228

>T142397 WP_003837806.1 NZ_CP045726:250232-250507 [Citrobacter freundii]
MKEKVLSLRKKQKNTLDQLFKAPTPQGIKWSEIESLIKALGGEIKEGRGSRCKFLLNKSIASFHRPHPSPDTDKGAVENV
RDWLTSIGVKP

Nucleotide

• Length: 276 bp

>T142397 NZ_CP060675:44889-46214 [Salmonella enterica]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13214.98 Da
• Isoelectric Point: 4.5462

>AT142397 WP_205688252.1 NZ_CP045726:250504-250863 [Citrobacter freundii]
MIKPKTPNSMEISGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGEISLREYLDDCSAAGIEPYANPEKLKT
FTLRYPESFGERLSFAAAEEQVSVNTWIIETLSKSLKQA

Nucleotide

• Length: 360 bp

>AT142397 NZ_CP060675:44591-44884 [Salmonella enterica]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0J1MPQ6