Detailed information of TA system TA142385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2085427..2087053
• Replicon: chromosome
• Accession: NZ_CP045720
• Organism: Pantoea eucalypti strain LMG 24197

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A501SHZ8
• Locus tag: EE896_RS09635
• Protein ID: WP_039660233.1
• Coordinates: 2085728..2087053 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: EE896_RS09630
• Protein ID: WP_003853607.1
• Coordinates: 2085427..2085723 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EE896_RS09615	2080902..2082284	+	1383	WP_140915596.1	M24 family metallopeptidase	-
EE896_RS09620	2082399..2084444	+	2046	WP_140915595.1	FUSC family protein	-
EE896_RS09625	2084784..2085119	-	336	WP_105099181.1	gamma-glutamylcyclotransferase	-
EE896_RS09630	2085427..2085723	+	297	WP_003853607.1	helix-turn-helix domain-containing protein	Antitoxin
EE896_RS09635	2085728..2087053	+	1326	WP_039660233.1	type II toxin-antitoxin system HipA family toxin	Toxin
EE896_RS09640	2087443..2088462	-	1020	WP_140915594.1	alpha/beta hydrolase	-
EE896_RS09645	2088953..2089375	+	423	WP_008925192.1	hydroxyisourate hydrolase	-
EE896_RS09650	2089392..2090183	-	792	Protein_1879	LysR family transcriptional regulator	-
EE896_RS09655	2090168..2090452	+	285	Protein_1880	SDR family oxidoreductase	-
EE896_RS09660	2090697..2091797	+	1101	WP_008925189.1	NADH:flavin oxidoreductase/NADH oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50169.09 Da
• Isoelectric Point: 6.0674

>T142385 WP_039660233.1 NZ_CP045720:2085728-2087053 [Pantoea eucalypti]
MSRRQQRLVIWMNGIQVGFWNKSKGEDTLQYLPEWSADAQGRPLSLSLPFTPGDQIWRGNVVRDYFDNLLPDSEGIRRRL
AMRYQAESLDPFDLLTELGKDCVGAIQLLKEGDEPTDLYCVKYRPLSESEIAMTLRNTTAASLPGRQSDTGDLRLSIAGA
QEKTALLWHDDRWCRPEGNTPTTHIFKLPLGLVGNMKADMSTSVENEWLCSLLLEQFGIPVARTEIAQFEDQKVLVVERF
DRKWSGDQRWIIRLPQEDMCQAAGMSPLRKYQADGGPGISEIMEILSHSDHAEQDRLHFFKAQIIFWVLAATDGHAKNFS
IAIGPQGHYCLTPLYDVLSAWPVIGNGNNQIAWQKCKLAMAARGSSNYYKLYRIQRRHWINHGEQTGLSKKQVEMMMDEI
ISDAPDVIERVRAQLPDQFPSELAERVFDGITQQCRRLAGN

Nucleotide

• Length: 1326 bp

>T142385 NZ_CP060674:c848724-848506 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10935.65 Da
• Isoelectric Point: 10.8315

>AT142385 WP_003853607.1 NZ_CP045720:2085427-2085723 [Pantoea eucalypti]
MKNVYPIKTLSQLRPLLIGFRKANGLTQKDLSERLGVTQQTYARLEANPARASIERLFKIFTLLGVEMTLSSGISSSAGK
PTEEKGTLENSPARREKW

Nucleotide

• Length: 297 bp

>AT142385 NZ_CP060674:c849126-848752 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A501SHZ8