Detailed information of TA system TA142366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 12933..13403
• Replicon: plasmid pFHI_NMBU_11_03
• Accession: NZ_CP045715
• Organism: Escherichia coli strain FHI_NMBU_11

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: GHT02_RS27140
• Protein ID: WP_000270923.1
• Coordinates: 12933..13211 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: -
• Locus tag: GHT02_RS27145
• Protein ID: WP_024175280.1
• Coordinates: 13215..13403 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT02_RS27100	7978..8286	+	309	WP_021526593.1	hypothetical protein	-
GHT02_RS27105	8352..9002	+	651	WP_001330913.1	hypothetical protein	-
GHT02_RS27110	9213..9698	+	486	WP_001061740.1	hypothetical protein	-
GHT02_RS27115	10511..10735	+	225	WP_050418674.1	hypothetical protein	-
GHT02_RS27120	10768..11118	+	351	WP_000694584.1	hypothetical protein	-
GHT02_RS27125	11438..12061	+	624	WP_001127156.1	ParA family protein	-
GHT02_RS27130	12058..12267	+	210	WP_000041404.1	hypothetical protein	-
GHT02_RS27135	12296..12787	+	492	WP_086218995.1	hypothetical protein	-
GHT02_RS27140	12933..13211	-	279	WP_000270923.1	virulence factor	Toxin
GHT02_RS27145	13215..13403	-	189	WP_024175280.1	DUF5397 family protein	Antitoxin
GHT02_RS27150	13524..13823	-	300	WP_021528494.1	XRE family transcriptional regulator	-
GHT02_RS27155	14195..14698	+	504	WP_021526598.1	hypothetical protein	-
GHT02_RS27160	14867..15235	+	369	WP_024186959.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34329	34329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (5-62)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10571.82 Da
• Isoelectric Point: 6.4606

>T142366 WP_000270923.1 NZ_CP045715:c13211-12933 [Escherichia coli]
MYAISFDLTVADTEKHHPKGVSQAYNDISSTLKRYGFERIQGSLYTNSDENMANLFQAMNALKALEWFPNSVRDIRAFRI
EQWSDFTSSIKG

Nucleotide

• Length: 279 bp

>T142366 NZ_CP060672:842913-843221 [Salmonella enterica]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6767.79 Da
• Isoelectric Point: 3.9020

>AT142366 WP_024175280.1 NZ_CP045715:c13403-13215 [Escherichia coli]
MNTVQIPTGTIKTFGLLGIPYEVGEPAQELPDGDILVNIILVQTGEKELYKLSKLLQDPDAE

Nucleotide

• Length: 189 bp

>AT142366 NZ_CP060672:842671-842910 [Salmonella enterica]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA