Detailed information of TA system TA142360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 18649..19256
• Replicon: plasmid pFHI_NMBU_11_02
• Accession: NZ_CP045714
• Organism: Escherichia coli strain FHI_NMBU_11

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A085NWP2
• Locus tag: GHT02_RS26470
• Protein ID: WP_000673981.1
• Coordinates: 18870..19256 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B7LIH9
• Locus tag: GHT02_RS26465
• Protein ID: WP_000493532.1
• Coordinates: 18649..18873 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT02_RS26425	14097..14519	-	423	WP_001255659.1	Exc2 family lipoprotein	-
GHT02_RS26430	14640..14918	+	279	WP_203048427.1	hypothetical protein	-
GHT02_RS26435	14910..15200	-	291	WP_000047032.1	hypothetical protein	-
GHT02_RS26440	15440..16936	+	1497	WP_001040753.1	hypothetical protein	-
GHT02_RS26445	16959..17492	-	534	WP_086218982.1	colicin immunity protein Cui	-
GHT02_RS26450	17544..17834	-	291	WP_001367320.1	colicin K immunity protein	-
GHT02_RS26455	17864..17941	+	78	WP_106904597.1	colicin release lysis protein	-
GHT02_RS26460	18272..18583	-	312	Protein_26	Tn3 family transposase	-
GHT02_RS26465	18649..18873	+	225	WP_000493532.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GHT02_RS26470	18870..19256	+	387	WP_000673981.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GHT02_RS26475	19619..20260	+	642	WP_001473476.1	recombinase family protein	-
GHT02_RS26480	20793..21218	-	426	WP_001563264.1	nucleoside triphosphatase NudI	-
GHT02_RS26485	21220..21927	-	708	WP_001563265.1	deoxynucleoside kinase	-
GHT02_RS26490	21924..22790	-	867	WP_064562334.1	radical SAM protein	-
GHT02_RS26495	23275..23526	+	252	WP_001563268.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GHT02_RS26500	23523..23810	+	288	WP_001563269.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..108688	108688
	-	flank	IS/Tn	-	-	19619..20260	641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14416.71 Da
• Isoelectric Point: 7.0732

>T142360 WP_000673981.1 NZ_CP045714:18870-19256 [Escherichia coli]
MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGIHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Nucleotide

• Length: 387 bp

>T142360 NZ_CP060671:17701-18006 [Salmonella enterica]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8361.39 Da
• Isoelectric Point: 5.6713

>AT142360 WP_000493532.1 NZ_CP045714:18649-18873 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Nucleotide

• Length: 225 bp

>AT142360 NZ_CP060671:17481-17699 [Salmonella enterica]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A085NWP2

Antitoxin

• AlphaFold DB: A0A086V9Y8