Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 4813..5360 Replicon plasmid pFHI_NMBU_11_02
Accession NZ_CP045714
Organism Escherichia coli strain FHI_NMBU_11

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag GHT02_RS26370 Protein ID WP_000969996.1
Coordinates 4813..5094 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID K4JYR0
Locus tag GHT02_RS26375 Protein ID WP_000079928.1
Coordinates 5091..5360 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHT02_RS26335 1..858 + 858 WP_001562860.1 incFII family plasmid replication initiator RepA -
GHT02_RS26340 1795..2124 + 330 WP_000493283.1 type II toxin-antitoxin system RelE/ParE family toxin -
GHT02_RS26345 2105..2386 + 282 WP_000780223.1 helix-turn-helix transcriptional regulator -
GHT02_RS26350 2569..2853 + 285 WP_024166087.1 ribbon-helix-helix domain-containing protein -
GHT02_RS26355 2853..3128 + 276 WP_000421250.1 type II toxin-antitoxin system RelE/ParE family toxin -
GHT02_RS26360 3320..3802 - 483 WP_001311056.1 hypothetical protein -
GHT02_RS26365 3919..4767 - 849 WP_001058009.1 3'-5' exonuclease -
GHT02_RS26370 4813..5094 - 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GHT02_RS26375 5091..5360 - 270 WP_000079928.1 hypothetical protein Antitoxin
GHT02_RS26380 5521..5760 + 240 WP_167549086.1 hypothetical protein -
GHT02_RS26385 5993..6355 + 363 Protein_11 3'-5' exonuclease -
GHT02_RS26390 6356..6714 + 359 Protein_12 ISKra4 family transposase -
GHT02_RS26395 6996..8135 + 1140 WP_001033384.1 helix-turn-helix domain-containing protein -
GHT02_RS26400 8255..9523 + 1269 WP_000253684.1 GntP family permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..108688 108688


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T142359 WP_000969996.1 NZ_CP045714:c5094-4813 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T142359 NZ_CP060670:4623295-4623582 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10175.50 Da        Isoelectric Point: 7.3210

>AT142359 WP_000079928.1 NZ_CP045714:c5360-5091 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT142359 NZ_CP060670:4623569-4623871 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A5W2J4D0

References