Detailed information of TA system TA142358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 2569..3128
• Replicon: plasmid pFHI_NMBU_11_02
• Accession: NZ_CP045714
• Organism: Escherichia coli strain FHI_NMBU_11

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A8S7NYT0
• Locus tag: GHT02_RS26355
• Protein ID: WP_000421250.1
• Coordinates: 2853..3128 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2T3SXK7
• Locus tag: GHT02_RS26350
• Protein ID: WP_024166087.1
• Coordinates: 2569..2853 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT02_RS26335	1..858	+	858	WP_001562860.1	incFII family plasmid replication initiator RepA	-
GHT02_RS26340	1795..2124	+	330	WP_000493283.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GHT02_RS26345	2105..2386	+	282	WP_000780223.1	helix-turn-helix transcriptional regulator	-
GHT02_RS26350	2569..2853	+	285	WP_024166087.1	ribbon-helix-helix domain-containing protein	Antitoxin
GHT02_RS26355	2853..3128	+	276	WP_000421250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHT02_RS26360	3320..3802	-	483	WP_001311056.1	hypothetical protein	-
GHT02_RS26365	3919..4767	-	849	WP_001058009.1	3'-5' exonuclease	-
GHT02_RS26370	4813..5094	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GHT02_RS26375	5091..5360	-	270	WP_000079928.1	hypothetical protein	-
GHT02_RS26380	5521..5760	+	240	WP_167549086.1	hypothetical protein	-
GHT02_RS26385	5993..6355	+	363	Protein_11	3'-5' exonuclease	-
GHT02_RS26390	6356..6714	+	359	Protein_12	ISKra4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..108688	108688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-74)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10525.39 Da
• Isoelectric Point: 9.5694

>T142358 WP_000421250.1 NZ_CP045714:2853-3128 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGLRLG
NVQYCTVALLR

Nucleotide

• Length: 276 bp

>T142358 NZ_CP060670:4579302-4579610 [Salmonella enterica]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10482.94 Da
• Isoelectric Point: 5.6558

>AT142358 WP_024166087.1 NZ_CP045714:2569-2853 [Escherichia coli]
IQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT142358 NZ_CP060670:4579060-4579299 [Salmonella enterica]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2T3SXK7