Detailed information of TA system TA142357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 72658..72998
• Replicon: plasmid pFHI_NMBU_11_01
• Accession: NZ_CP045713
• Organism: Escherichia coli strain FHI_NMBU_11

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: GHT02_RS26170
• Protein ID: WP_000969988.1
• Coordinates: 72816..72998 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: GHT02_RS26165
• Protein ID: WP_001376226.1
• Coordinates: 72658..72819 (+)
• Length: 54 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT02_RS26150	67781..68101	-	321	WP_000668718.1	hypothetical protein	-
GHT02_RS26155	68809..71847	-	3039	WP_086218989.1	Tn3 family transposase	-
GHT02_RS26160	72004..72585	+	582	WP_001196199.1	recombinase family protein	-
GHT02_RS26165	72658..72819	+	162	WP_001376226.1	hypothetical protein	Antitoxin
GHT02_RS26170	72816..72998	+	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHT02_RS26175	73097..73405	+	309	WP_000377483.1	hypothetical protein	-
GHT02_RS26180	73965..74999	+	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC	1..114161	114161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-65)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T142357 WP_000969988.1 NZ_CP045713:72816-72998 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T142357 NZ_CP060670:4538201-4538485 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 54 a.a.
• Molecular weight: 6224.92 Da
• Isoelectric Point: 7.2038

>AT142357 WP_001376226.1 NZ_CP045713:72658-72819 [Escherichia coli]
MRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 162 bp

>AT142357 NZ_CP060670:4537969-4538211 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3