Detailed information of TA system TA142346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4253694..4254319
• Replicon: chromosome
• Accession: NZ_CP045712
• Organism: Escherichia coli strain FHI_NMBU_11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GHT02_RS20660
• Protein ID: WP_000911330.1
• Coordinates: 4253921..4254319 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GHT02_RS20655
• Protein ID: WP_000450524.1
• Coordinates: 4253694..4253921 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT02_RS20630	4249497..4249967	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
GHT02_RS20635	4249967..4250539	-	573	WP_000176192.1	glycine cleavage system transcriptional repressor	-
GHT02_RS20640	4250685..4251563	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GHT02_RS20645	4251580..4252614	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
GHT02_RS20650	4252827..4253540	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GHT02_RS20655	4253694..4253921	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GHT02_RS20660	4253921..4254319	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GHT02_RS20665	4254466..4255329	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
GHT02_RS20670	4255344..4257359	+	2016	WP_000829388.1	tRNA cytosine(34) acetyltransferase TmcA	-
GHT02_RS20675	4257433..4258131	+	699	WP_000679812.1	esterase	-
GHT02_RS20680	4258212..4258412	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T142346 WP_000911330.1 NZ_CP045712:4253921-4254319 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T142346 NZ_CP060670:1235094-1235197 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT142346 WP_000450524.1 NZ_CP045712:4253694-4253921 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT142346 NZ_CP060670:c1235199-1235054 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33