Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 3153860..3154660 Replicon chromosome
Accession NZ_CP045712
Organism Escherichia coli strain FHI_NMBU_11

Toxin (Protein)


Gene name ataT Uniprot ID -
Locus tag GHT02_RS15065 Protein ID WP_000342454.1
Coordinates 3154133..3154660 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag GHT02_RS15060 Protein ID WP_001277108.1
Coordinates 3153860..3154126 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHT02_RS15040 3149517..3150185 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
GHT02_RS15045 3150178..3151236 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
GHT02_RS15050 3151481..3152335 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
GHT02_RS15055 3152606..3153709 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
GHT02_RS15060 3153860..3154126 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
GHT02_RS15065 3154133..3154660 + 528 WP_000342454.1 GNAT family N-acetyltransferase Toxin
GHT02_RS15070 3154657..3155040 - 384 WP_000778814.1 aspartate 1-decarboxylase autocleavage activator PanM -
GHT02_RS15075 3155464..3156573 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
GHT02_RS15080 3156621..3157547 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
GHT02_RS15085 3157544..3158821 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
GHT02_RS15090 3158818..3159585 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(90-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19722.66 Da        Isoelectric Point: 6.9585

>T142341 WP_000342454.1 NZ_CP045712:3154133-3154660 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVMGYYTLSGSCFERVALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T142341 NZ_CP060668:c4524822-4524331 [Salmonella enterica]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT142341 WP_001277108.1 NZ_CP045712:3153860-3154126 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT142341 NZ_CP060668:c4525112-4524819 [Salmonella enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References