Detailed information of TA system TA142340

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2789635..2790373
• Replicon: chromosome
• Accession: NZ_CP045712
• Organism: Escherichia coli strain FHI_NMBU_11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H0JYG3
• Locus tag: GHT02_RS13420
• Protein ID: WP_001094432.1
• Coordinates: 2789635..2790012 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GHT02_RS13425
• Protein ID: WP_074431268.1
• Coordinates: 2790059..2790373 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT02_RS13395	2786217..2786345	-	129	Protein_2610	virulence RhuM family protein	-
GHT02_RS13400	2786579..2787424	-	846	WP_001280531.1	DUF4942 domain-containing protein	-
GHT02_RS13405	2787708..2788727	-	1020	WP_000875213.1	IS110 family transposase	-
GHT02_RS13410	2788941..2789138	-	198	WP_000839249.1	DUF957 domain-containing protein	-
GHT02_RS13415	2789150..2789638	-	489	WP_000779178.1	hypothetical protein	-
GHT02_RS13420	2789635..2790012	-	378	WP_001094432.1	TA system toxin CbtA family protein	Toxin
GHT02_RS13425	2790059..2790373	-	315	WP_074431268.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GHT02_RS13430	2790512..2790733	-	222	WP_000692313.1	DUF987 domain-containing protein	-
GHT02_RS13435	2790802..2791278	-	477	WP_001186704.1	RadC family protein	-
GHT02_RS13440	2791293..2791766	-	474	WP_000855069.1	antirestriction protein	-
GHT02_RS13445	2791900..2792100	-	201	WP_001367430.1	hypothetical protein	-
GHT02_RS13450	2792100..2792921	-	822	WP_001241714.1	DUF945 domain-containing protein	-
GHT02_RS13455	2793001..2793212	+	212	Protein_2622	hypothetical protein	-
GHT02_RS13460	2793324..2793785	-	462	WP_000581501.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2787708..2788727	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (2-96)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14136.99 Da
• Isoelectric Point: 6.4729

>T142340 WP_001094432.1 NZ_CP045712:c2790012-2789635 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T142340 NZ_CP060668:c4417719-4417435 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11312.06 Da
• Isoelectric Point: 6.4613

>AT142340 WP_074431268.1 NZ_CP045712:c2790373-2790059 [Escherichia coli]
MPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEA
DTLGSCGYVYLAVYPTPAAPAITV

Nucleotide

• Length: 315 bp

>AT142340 NZ_CP060668:c4417951-4417709 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0H0JYG3