Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2249471..2249968 Replicon chromosome
Accession NZ_CP045712
Organism Escherichia coli strain FHI_NMBU_11

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GHT02_RS10925 Protein ID WP_001173343.1
Coordinates 2249471..2249644 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GHT02_RS10930 Protein ID WP_000398619.1
Coordinates 2249672..2249968 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GHT02_RS10915 2246141..2247598 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
GHT02_RS10920 2247835..2249352 + 1518 WP_001295074.1 lysine--tRNA ligase -
GHT02_RS10925 2249471..2249644 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GHT02_RS10930 2249672..2249968 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GHT02_RS10935 2250195..2250467 - 273 WP_000405647.1 N-acetyltransferase -
GHT02_RS10940 2250479..2250709 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
GHT02_RS10945 2250890..2252521 + 1632 WP_001216487.1 sensor histidine kinase -
GHT02_RS10950 2252518..2253237 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2202683..2249968 47285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T142339 WP_001173343.1 NZ_CP045712:c2249644-2249471 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T142339 NZ_CP060668:c4376618-4376310 [Salmonella enterica]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT142339 WP_000398619.1 NZ_CP045712:c2249968-2249672 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT142339 NZ_CP060668:c4376860-4376621 [Salmonella enterica]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References