Detailed information of TA system TA142338

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2236635..2237433
• Replicon: chromosome
• Accession: NZ_CP045712
• Organism: Escherichia coli strain FHI_NMBU_11

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0P7PH46
• Locus tag: GHT02_RS10870
• Protein ID: WP_000854896.1
• Coordinates: 2237056..2237433 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A234XUW5
• Locus tag: GHT02_RS10865
• Protein ID: WP_024175300.1
• Coordinates: 2236635..2237009 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHT02_RS10840	2233979..2234212	+	234	WP_001117568.1	DUF905 family protein	-
GHT02_RS10845	2234303..2235121	+	819	WP_033884686.1	DUF945 domain-containing protein	-
GHT02_RS10850	2235211..2235696	+	486	WP_000214386.1	antirestriction protein	-
GHT02_RS10855	2235712..2236188	+	477	WP_001186727.1	RadC family protein	-
GHT02_RS10860	2236251..2236472	+	222	WP_000692358.1	DUF987 domain-containing protein	-
GHT02_RS10865	2236635..2237009	+	375	WP_024175300.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GHT02_RS10870	2237056..2237433	+	378	WP_000854896.1	TA system toxin CbtA family protein	Toxin
GHT02_RS10875	2237430..2237918	+	489	WP_000761662.1	hypothetical protein	-
GHT02_RS10880	2237930..2238127	+	198	WP_000839256.1	DUF957 domain-containing protein	-
GHT02_RS10885	2238212..2239078	+	867	WP_000014510.1	DUF4942 domain-containing protein	-
GHT02_RS10890	2239150..2239413	+	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
GHT02_RS10895	2239410..2239736	+	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GHT02_RS10900	2240617..2242155	+	1539	WP_001187200.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2202683..2249968	47285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14210.19 Da
• Isoelectric Point: 7.2920

>T142338 WP_000854896.1 NZ_CP045712:2237056-2237433 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T142338 NZ_CP060668:c4332625-4332338 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13664.42 Da
• Isoelectric Point: 6.4757

>AT142338 WP_024175300.1 NZ_CP045712:2236635-2237009 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVKPCFGARLVQEGNRLHYLADRAGISGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHSVTLYAKGLTCEADTLGSCGYVYLTVYPTPATATTS

Nucleotide

• Length: 375 bp

>AT142338 NZ_CP060668:c4332351-4332049 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A0P7PH46

Antitoxin

• AlphaFold DB: A0A234XUW5