Detailed information of TA system TA142331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1235386..1236303
• Replicon: chromosome
• Accession: NZ_CP045711
• Organism: Bacillus velezensis strain HN-Q-8

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: GG619_RS06450
• Protein ID: WP_007407256.1
• Coordinates: 1235557..1236303 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: GG619_RS06445
• Protein ID: WP_003154807.1
• Coordinates: 1235386..1235556 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GG619_RS06410	1230609..1232240	+	1632	WP_015417294.1	pyocin knob domain-containing protein	-
GG619_RS06415	1232253..1232624	+	372	WP_015417295.1	YomQ/XkdW protein, phage-like element PBSX	-
GG619_RS06420	1232629..1232826	+	198	WP_012117366.1	XkdX family protein	-
GG619_RS06425	1232883..1233644	+	762	WP_156240322.1	phage portal protein	-
GG619_RS06430	1233696..1233959	+	264	WP_031378810.1	hemolysin XhlA family protein	-
GG619_RS06435	1233973..1234236	+	264	WP_003154813.1	phage holin	-
GG619_RS06440	1234250..1235128	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
GG619_RS06445	1235386..1235556	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
GG619_RS06450	1235557..1236303	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
GG619_RS06455	1236407..1237405	-	999	WP_003154805.1	inorganic phosphate transporter	-
GG619_RS06460	1237418..1238035	-	618	WP_003154804.1	DUF47 domain-containing protein	-
GG619_RS06465	1238321..1239637	-	1317	WP_007610842.1	amino acid permease	-
GG619_RS06470	1239960..1240910	+	951	WP_031378808.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T142331 WP_007407256.1 NZ_CP045711:c1236303-1235557 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T142331 NZ_CP060668:1171935-1172348 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT142331 WP_003154807.1 NZ_CP045711:c1235556-1235386 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT142331 NZ_CP060668:1171688-1171954 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14