Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 493152..493789 Replicon chromosome
Accession NZ_CP045711
Organism Bacillus velezensis strain HN-Q-8

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GG619_RS02510 Protein ID WP_003156187.1
Coordinates 493439..493789 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag GG619_RS02505 Protein ID WP_003156188.1
Coordinates 493152..493433 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GG619_RS02485 489517..490116 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
GG619_RS02490 490209..490574 + 366 WP_007410229.1 holo-ACP synthase -
GG619_RS02495 490739..491746 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
GG619_RS02500 491863..493032 + 1170 WP_061890435.1 alanine racemase -
GG619_RS02505 493152..493433 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GG619_RS02510 493439..493789 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GG619_RS02515 493907..494728 + 822 WP_015416874.1 STAS domain-containing protein -
GG619_RS02520 494733..495098 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
GG619_RS02525 495101..495502 + 402 WP_003156178.1 anti-sigma regulatory factor -
GG619_RS02530 495514..496521 + 1008 WP_015416875.1 PP2C family protein-serine/threonine phosphatase -
GG619_RS02535 496585..496914 + 330 WP_033575044.1 anti-sigma factor antagonist -
GG619_RS02540 496911..497393 + 483 WP_007609591.1 anti-sigma B factor RsbW -
GG619_RS02545 497359..498147 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
GG619_RS02550 498147..498749 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T142330 WP_003156187.1 NZ_CP045711:493439-493789 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T142330 NZ_CP060668:704811-705086 [Salmonella enterica]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT142330 WP_003156188.1 NZ_CP045711:493152-493433 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT142330 NZ_CP060668:704548-704808 [Salmonella enterica]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References