Detailed information of TA system TA142328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1577313..1577998
• Replicon: chromosome
• Accession: NZ_CP045707
• Organism: Neisseria gonorrhoeae strain NG250

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q5F6D1
• Locus tag: GHB96_RS08060
• Protein ID: WP_003689143.1
• Coordinates: 1577816..1577998 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q5F6D2
• Locus tag: GHB96_RS08055
• Protein ID: WP_003691454.1
• Coordinates: 1577313..1577714 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHB96_RS08000 (GHB96_08495)	1572357..1572572	-	216	WP_003691538.1	hypothetical protein	-
GHB96_RS08005 (GHB96_08500)	1572624..1573115	-	492	WP_047916888.1	siphovirus Gp157 family protein	-
GHB96_RS08010 (GHB96_08505)	1573112..1573294	-	183	WP_003691535.1	hypothetical protein	-
GHB96_RS08015 (GHB96_08510)	1573434..1574120	-	687	WP_010357532.1	hypothetical protein	-
GHB96_RS08020 (GHB96_08515)	1574189..1574350	-	162	WP_003693867.1	hypothetical protein	-
GHB96_RS08025 (GHB96_08520)	1574347..1574622	-	276	WP_082295533.1	hypothetical protein	-
GHB96_RS08030 (GHB96_08525)	1574775..1575107	-	333	WP_050155386.1	hypothetical protein	-
GHB96_RS08035 (GHB96_08530)	1575249..1575536	-	288	WP_215772512.1	hypothetical protein	-
GHB96_RS08040 (GHB96_08535)	1575533..1576009	-	477	WP_002255718.1	hypothetical protein	-
GHB96_RS08045 (GHB96_08540)	1576042..1576242	-	201	WP_003704298.1	hypothetical protein	-
GHB96_RS08050 (GHB96_08545)	1576453..1577214	+	762	WP_012503753.1	hypothetical protein	-
GHB96_RS08055 (GHB96_08550)	1577313..1577714	-	402	WP_003691454.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
GHB96_RS08060 (GHB96_08555)	1577816..1577998	-	183	WP_003689143.1	type II toxin-antitoxin system HicA family toxin	Toxin
GHB96_RS08065 (GHB96_08560)	1578168..1578986	-	819	WP_012503752.1	DUF3037 domain-containing protein	-
GHB96_RS08070 (GHB96_08565)	1578983..1579681	-	699	WP_003689139.1	hypothetical protein	-
GHB96_RS08075 (GHB96_08570)	1579920..1580630	-	711	WP_050161643.1	helix-turn-helix transcriptional regulator	-
GHB96_RS08080 (GHB96_08575)	1580746..1580934	+	189	WP_003689136.1	helix-turn-helix domain-containing protein	-
GHB96_RS08085 (GHB96_08580)	1581014..1581169	+	156	WP_003689578.1	hypothetical protein	-
GHB96_RS08090 (GHB96_08585)	1581146..1581334	-	189	WP_003691445.1	hypothetical protein	-
GHB96_RS08095 (GHB96_08590)	1581507..1581734	+	228	WP_003698261.1	helix-turn-helix domain-containing protein	-
GHB96_RS11975	1581912..1582340	+	429	WP_252345276.1	hypothetical protein	-
GHB96_RS11980	1582435..1582938	+	504	WP_010360005.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1562129..1596419	34290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6687.77 Da
• Isoelectric Point: 10.7235

>T142328 WP_003689143.1 NZ_CP045707:c1577998-1577816 [Neisseria gonorrhoeae]
MNSLDVIALLKQDGWYKVAQSGSHSQYKHPTKKGRVTVPHPKKDLPTGTVKNIYKQAGLK

Nucleotide

• Length: 183 bp

>T142328 NZ_CP060668:546320-546805 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGTGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14634.43 Da
• Isoelectric Point: 4.5457

>AT142328 WP_003691454.1 NZ_CP045707:c1577714-1577313 [Neisseria gonorrhoeae]
MFIPAALHKDEHSAYGVTIPDLPGCFSCGDTVEEAVANARSAAYMHIDGMIEDGGFKNLAVSSIADLSQEPDYHGATWVM
IEIDPAKISRQQIRFNVSWPQYLLDRVDEYTSANHETRSGFLAKAALLTMNQA

Nucleotide

• Length: 402 bp

>AT142328 NZ_CP060668:546045-546332 [Salmonella enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: Q5F6D1

Antitoxin

• AlphaFold DB: Q5F6D2