Detailed information of TA system TA142315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 427959..428608
• Replicon: chromosome
• Accession: NZ_CP045701
• Organism: Pseudomonas brassicacearum strain S-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A5Q0S205
• Locus tag: GFU70_RS01870
• Protein ID: WP_058546213.1
• Coordinates: 427959..428378 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A923FFX2
• Locus tag: GFU70_RS01875
• Protein ID: WP_058546214.1
• Coordinates: 428378..428608 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFU70_RS01845	423158..424390	-	1233	WP_153387516.1	methyltransferase	-
GFU70_RS01850	424371..425060	-	690	WP_116643371.1	ABC transporter permease	-
GFU70_RS01855	425057..425752	-	696	WP_064106967.1	ABC transporter permease	-
GFU70_RS01860	425831..426598	-	768	WP_058546212.1	ABC transporter substrate-binding protein	-
GFU70_RS01865	426612..427385	-	774	WP_003196795.1	ABC transporter ATP-binding protein	-
GFU70_RS01870	427959..428378	-	420	WP_058546213.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GFU70_RS01875	428378..428608	-	231	WP_058546214.1	antitoxin	Antitoxin
GFU70_RS01880	428793..429242	+	450	WP_153387517.1	carboxypeptidase-like regulatory domain-containing protein	-
GFU70_RS01885	429252..429698	-	447	WP_116643368.1	hypothetical protein	-
GFU70_RS01890	429739..431973	-	2235	WP_153387518.1	GAF domain-containing protein	-
GFU70_RS01895	431970..432575	-	606	WP_153387519.1	biliverdin-producing heme oxygenase	-
GFU70_RS01900	432670..433320	-	651	WP_153387520.1	DUF1016 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	407956..428608	20652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15314.96 Da
• Isoelectric Point: 7.2917

>T142315 WP_058546213.1 NZ_CP045701:c428378-427959 [Pseudomonas brassicacearum]
MIKFMLDTNICIFTIKNKPQIVREAFNLHHGQLCISAVTLMELIYGAEKSSAPEKNLGVIEGFCARLEVLPFGYDAAAHT
GMIRAELAKIGKPIGPYDQMIAGHARSLGLIVVTNNLKEFERVPGLRIEDWTTVTTTGL

Nucleotide

• Length: 420 bp

>T142315 NZ_CP060666:c3629632-3629345 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8503.52 Da
• Isoelectric Point: 4.3719

>AT142315 WP_058546214.1 NZ_CP045701:c428608-428378 [Pseudomonas brassicacearum]
MEQTTLFMSNRSQAVRLPKAVAMPSDVKRVDVIAIGRARIITPAGEAWDSWFDGDSATADFMAEREQPADQERESF

Nucleotide

• Length: 231 bp

>AT142315 NZ_CP060666:c3629358-3629056 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A5Q0S205