Detailed information of TA system TA142308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 351011..351570
• Replicon: chromosome
• Accession: NZ_CP045697
• Organism: Brucella sp. 2280

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: GHC20_RS01755
• Protein ID: WP_004688321.1
• Coordinates: 351011..351256 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: GHC20_RS01760
• Protein ID: WP_153526797.1
• Coordinates: 351256..351570 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHC20_RS01735	346461..347696	-	1236	WP_153527993.1	phage portal protein	-
GHC20_RS01740	347711..349183	-	1473	WP_210354517.1	terminase large subunit	-
GHC20_RS01745	349399..349800	-	402	WP_025200428.1	hypothetical protein	-
GHC20_RS01750	350613..350924	-	312	WP_153526795.1	hypothetical protein	-
GHC20_RS01755	351011..351256	+	246	WP_004688321.1	BrnT family toxin	Toxin
GHC20_RS01760	351256..351570	+	315	WP_153526797.1	BrnA antitoxin family protein	Antitoxin
GHC20_RS01765	351571..351840	-	270	WP_153526799.1	hypothetical protein	-
GHC20_RS01770	352404..352982	-	579	WP_153526801.1	hypothetical protein	-
GHC20_RS01775	353005..353193	-	189	WP_153526803.1	hypothetical protein	-
GHC20_RS01780	353203..353679	-	477	WP_153526805.1	hypothetical protein	-
GHC20_RS01785	353679..353903	-	225	WP_153526807.1	hypothetical protein	-
GHC20_RS01790	353900..355018	-	1119	WP_153526809.1	helix-turn-helix domain-containing protein	-
GHC20_RS01795	355015..355371	-	357	WP_153526811.1	DUF1064 domain-containing protein	-
GHC20_RS01800	355368..355610	-	243	WP_153526813.1	hypothetical protein	-
GHC20_RS01805	355582..355830	-	249	WP_153526815.1	hypothetical protein	-
GHC20_RS01810	355926..356354	-	429	WP_153526817.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	318955..368128	49173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T142308 WP_004688321.1 NZ_CP045697:351011-351256 [Brucella sp. 2280]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T142308 NZ_CP060666:466399-466812 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11666.48 Da
• Isoelectric Point: 10.3131

>AT142308 WP_153526797.1 NZ_CP045697:351256-351570 [Brucella sp. 2280]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKVIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT142308 NZ_CP060666:466152-466418 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA